Organic compounds

ABSTRACT

Optionally substituted 4,5,7,8-tetrahydro-(optionally 4-oxo, 4-thioxo or 4-imino)-2H-imidazo[1,2-a]pyrrolo[3,4-e]pyrimidine or 4,5,7,8,9-pentahydro-(optionally 4-oxo, 4-thioxo or 4-imino)-2H-pyrimido[1,2-a]pyrrolo[3,4-e]pyrimidine compounds or Compounds of Formula (I), processes for their production, their use as pharmaceuticals and pharmaceutical compositions comprising the same.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a U.S. filing under 35 U.S.C. 371 of PCT/US2009/06438 filed on Dec. 7, 2009, which claims the benefit of U.S. Provisional Application No. 61/120,440 filed on Dec. 6, 2008, the contents of each of which are incorporated herein by reference.

TECHNICAL FIELD

The present invention relates to optionally substituted 4,5,7,8-tetrahydro-(optionally 4-oxo, 4-thioxo or 4-imino)-2H-imidazo[1,2-a]pyrrolo[3,4-e]pyrimidine or 4,5,7,8,9-pentahydro-(optionally 4-oxo, 4-thioxo or 4-imino)-2H-pyrimido[1,2-a]pyrrolo[3,4-e]pyrimidine, for example, compounds of Formula II (Formula II-A and II-B) and Formula I (Formula I-A and I-B) as described below, processes for their production, their use as pharmaceuticals and pharmaceutical compositions comprising them. Of particular interest are novel compounds useful as inhibitors of phosphodiesterase 1 (PDE1), e.g., in the treatment of diseases involving disorders of the dopamine D1 receptor intracellular pathway, such as Parkinson's disease, depression, narcolepsy, damage to cognitive function, e.g., in schizophrenia, or disorders that may be ameliorated through enhanced progesterone-signaling pathway, e.g., female sexual dysfunction as well as other disease or conditions characterized by low levels of cAMP and/or cGMP in cells expressing PDE1 and those characterized by reduced dopamine D1 receptor signaling activities.

BACKGROUND OF THE INVENTION

Eleven families of phosphodiesterases (PDEs) have been identified but only PDEs in Family I, the Ca²⁺-calmodulin-dependent phosphodiesterases (CaM-PDEs), have been shown to mediate both the calcium and cyclic nucleotide (e.g. cAMP and cGMP) signaling pathways. The three known CaM-PDE genes, PDE1A, PDE1B, and PDE1C, are all expressed in central nervous system tissue. PDE1A is expressed throughout the brain with higher levels of expression in the CA1 to CA3 layers of the hippocampus and cerebellum and at a low level in the striatum. PDE1A is also expressed in the lung and heart. PDE1B is predominately expressed in the striatum, dentate gyms, olfactory tract and cerebellum, and its expression correlates with brain regions having high levels of dopaminergic innervation. Although PDE1B is primarily expressed in the central nervous system, it may be detected in the heart. PDE1C is primarily expressed in olfactory epithelium, cerebellar granule cells, and striatum. PDE1C is also expressed in the heart and vascular smooth muscle.

Cyclic nucleotide phosphodiesterases decrease intracellular cAMP and cGMP signaling by hydrolyzing these cyclic nucleotides to their respective inactive 5′-monophosphates (5′AMP and 5′GMP). CaM-PDEs play a critical role in mediating signal transduction in brain cells, particularly within an area of the brain known as the basal ganglia or striatum. For example, NMDA-type glutamate receptor activation and/or dopamine D2 receptor activation result in increased intracellular calcium concentrations, leading to activation of effectors such as calmodulin-dependent kinase II (CaMKII) and calcineurin and to activation of CaM-PDEs, resulting in reduced cAMP and cGMP. Dopamine D1 receptor activation, on the other hand, leads to activation of nucleotide cyclases, resulting in increased cAMP and cGMP. These cyclic nucleotides in turn activate protein kinase A (PKA; cAMP-dependent protein kinase) and/or protein kinase G (PKG; cGMP-dependent protein kinase) that phosphorylate downstream signal transduction pathway elements such as DARPP-32 (dopamine and cAMP-regulated phosphoprotein) and cAMP responsive element binding protein (CREB). Phosphorylated DARPP-32 in turn inhibits the activity of protein phosphates-1 (PP-1), thereby increasing the state of phosphorylation of substrate proteins such as progesterone receptor (PR), leading to induction of physiologic responses. Studies in rodents have suggested that inducing cAMP and cGMP synthesis through activation of dopamine D1 or progesterone receptor enhances progesterone signaling associated with various physiological responses, including the lordosis response associated with receptivity to mating in some rodents. See Mani, et al., Science (2000) 287: 1053, the contents of which are incorporated herein by reference.

CaM-PDEs can therefore affect dopamine-regulated and other intracellular signaling pathways in the basal ganglia (striatum), including but not limited to nitric oxide, noradrenergic, neurotensin, CCK, VIP, serotonin, glutamate (e.g., NMDA receptor, AMPA receptor), GABA, acetylcholine, adenosine (e.g., A2A receptor), cannabinoid receptor, natriuretic peptide (e.g., ANP, BNP, CNP), DARPP-32, and endorphin intracellular signaling pathways.

Phosphodiesterase (PDE) activity, in particular, phosphodiesterase 1 (PDE1) activity, functions in brain tissue as a regulator of locomotor activity and learning and memory. PDE1 is a therapeutic target for regulation of intracellular signaling pathways, preferably in the nervous system, including but not limited to a dopamine D1 receptor, dopamine D2 receptor, nitric oxide, noradrenergic, neurotensin, CCK, VIP, serotonin, glutamate (e.g., NMDA receptor, AMPA receptor), GABA, acetylcholine, adenosine (e.g., A2A receptor), cannabinoid receptor, natriuretic peptide (e.g., ANP, BNP, CNP), endorphin intracellular signaling pathway and progesterone signaling pathway. For example, inhibition of PDE1B should act to potentiate the effect of a dopamine D1 agonist by protecting cGMP and cAMP from degradation, and should similarly inhibit dopamine D2 receptor signaling pathways, by inhibiting PDE1 activity. Chronic elevation in intracellular calcium levels is linked to cell death in numerous disorders, particularly in neurodegerative diseases such as Alzheimer's, Parkinson's and Huntington's Diseases and in disorders of the circulatory system leading to stroke and myocardial infarction. PDE1 inhibitors are therefore potentially useful in diseases characterized by reduced dopamine D1 receptor signaling activity, such as Parkinson's disease, restless leg syndrome, depression, narcolepsy and cognitive impairment. PDE1 inhibitors are also useful in diseases that may be alleviated by the enhancement of progesterone-signaling such as female sexual dysfunction.

There is thus a need for compounds that selectively inhibit PDE1 activity, especially PDE1A and/or PDE1B activity.

SUMMARY OF THE INVENTION

The invention provides optionally substituted 4,5,7,8-tetrahydro-2H-imidazo[1,2-a]pyrrolo[3,4-e]pyrimidine or 4,5,7,8,9-pentahydro-2H-pyrimido[1,2-a]pyrrolo[3,4-e]pyrimidine, e.g., a Compound of Formula II, e.g., II-A or II-B:

wherein

-   -   (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂;     -   (ii) L is a single bond, —N(H)—, —CH₂—, —S—, —S(O)— or —S(O₂)—;     -   (iii) R₁ is H or C₁₋₄ alkyl (e.g., methyl);     -   (iv) R₄ is H or C₁₋₆ alkyl (e.g., methyl or isopropyl) and R₂         and R₃ are, independently,         -   H         -   C₁₋₆alkyl (e.g., methyl, isopropyl) optionally substituted             with halo or hydroxy (e.g., R₂ and R₃ are both methyl, or R₂             is H and R₃ is methyl, ethyl, isopropyl or hydroxyethyl),         -   aryl,         -   heteroaryl,         -   (optionally hetero)arylalkoxy,         -   (optionally hetero)arylC₁₋₆alkyl; or         -   R₂ and R₃ together form a 3- to 6-membered ring;         -   or         -   R₂ is H and R₃ and R₄ together form a di-, tri- or             tetramethylene bridge (pref. wherein the R₃ and R₄ together             have the cis configuration, e.g., where the carbons carrying             R₃ and R₄ have the R and S configurations, respectively);         -   or     -   (v) R₅ is         -   a) -D-E-F, wherein:             -   D is C₁₋₄alkylene (e.g., methylene, ethylene or                 prop-2-yn-1-ylene);             -   E is a single bond, C₂₋₄alkynylene (e.g., arylene (e.g.,                 phenylene) or heteroarylene (e.g., pyridylene);             -   F is                 -   H,                 -   aryl (e.g., phenyl),                 -   heteroaryl (e.g., pyridyl, diazolyl, triazolyl, for                     example, pyrid-2-yl, imidazol-1-yl,                     1,2,4-triazol-1-yl),                 -   halo (e.g., F, Br, Cl),                 -   haloC₁₋₄alkyl (e.g., trifluoromethyl),                 -   —C(O)—R₁₅,                 -   —N(R₁₆)(R₁₇), or                 -   C₃₋₇cycloalkyl optionally containing at least one                     atom selected from a group consisting of N or O                     (e.g., cyclopentyl, cyclohexyl, pyrrolidinyl (e.g.,                     pyrrolidin-3-yl), tetrahydro-2H-pyran-4-yl, or                     morpholinyl);             -   wherein D, E and F are independently and optionally                 substituted with one or more halo (e.g., F, Cl or Br),                 C₁₋₄alkyl (e.g., methyl), haloC₁₋₄alkyl (e.g.,                 trifluoromethyl), C₁₋₄alkoxy (e.g., methoxy), hydroxy,                 C₁₋₄carboxy, or an additional aryl or heteroaryl (e.g.,                 biphenyl or pyridylphenyl),             -   for example, F is heteroaryl, e.g., pyridyl substituted                 with one or more halo (e.g., 6-fluoropyrid-2-yl,                 5-fluoropyrid-2-yl, 6-fluoropyrid-2-yl,                 3-fluoropyrid-2-yl, 4-fluoropyrid-2-yl,                 4,6-dichloropyrid-2-yl), haloC₁₋₄alkyl (e.g.,                 5-trifluoromethylpyrid-2-yl) or C₁₋₄alkyl (e.g.,                 5-methylpyrid-2-yl), or F is aryl, e.g., phenyl,                 substituted with one or more halo (e.g., 4-fluorophenyl)                 or F is a C₃₋₇heterocycloalkyl (e.g., pyrrolidinyl)                 optionally substituted with a C₁₋₆alkyl (e.g.,                 1-methylpyrrolidin-3-yl); or         -   b) a substituted heteroarylalkyl, e.g., substituted with             haloC₁₋₄alkyl;         -   c) attached to the nitrogen on the pyrrolo portion of             Formula II-A or II-B and is a moiety of Formula A

-   -   -   wherein X, Y and Z are, independently, N or C, and R₈, R₉,             R₁₁ and R₁₂ are independently H or halogen (e.g., Cl or F),             and R₁₀ is             -   halogen,             -   C₁₋₄alkyl,             -   haloC₁₋₄alkyl (e.g., triflouromethyl)             -   C₁₋₄alkoxy (e.g. methoxy),             -   C₃₋₇cycloalkyl,             -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl or                 piperidinyl),             -   C₁₋₄haloalkyl (e.g., trifluoromethyl),             -   aryl (e.g., phenyl),             -   heteroaryl (e.g., pyridyl (for example pyrid-2-yl or                 pyrid-4-yl), or thiadiazolyl (e.g.,                 1,2,3-thiadiazol-4-yl)), diazolyl (e.g., imidazol-1-yl),                 triazolyl (e.g., 1,2,4-triazol-1-yl), tetrazolyl,             -   arylcarbonyl (e.g., benzoyl),             -   alkylsulfonyl (e.g., methylsulfonyl),             -   heteroarylcarbonyl, or             -   alkoxycarbonyl;         -   wherein the aryl, heteroaryl, cycloalkyl or heterocycloalkyl             is independently, optionally substituted with one or more             C₁₋₄alkyl (e.g., methyl), halogen (e.g., chloro or fluoro),             haloC₁₋₄alkyl (e.g., trifluoromethyl), hydroxy, C₁₋₄carboxy,             —SH or an additional aryl, heteroaryl (e.g., biphenyl or             pyridylphenyl) or C₃₋₈cycloalkyl,         -   preferably R₁₀ is phenyl, pyridyl, piperidinyl or             pyrrolidinyl optionally substituted with the substituents             previously defined, e.g. optionally substituted with halo or             alkyl         -   provided that when X, Y, or Z is nitrogen, R₈, R₉, or R₁₀,             respectively, is not present;

    -   (vi) R₆ is         -   H,         -   C₁₋₄alkyl (e.g., methyl, ethyl, n-propyl, isobutyl),         -   C₃₋₇cycloalkyl (e.g., cyclopentyl or cyclohexyl),         -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl, piperidinyl,             morpholinyl),         -   aryl (e.g., phenyl),         -   heteroaryl (e.g., pyrid-4-yl),         -   arylC₁₋₄alkyl (e.g., benzyl),         -   arylamino (e.g., phenylamino),         -   heteroarylamino,         -   N,N-diC₁₋₄alkylamino,         -   N,N-diarylamino,         -   N-aryl-N-(arylC₁₋₄alkyl)amino (e.g.,             N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino), or         -   —N(R₁₈)(R₁₉),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;

    -   (vii) R₇ is H, C₁₋₆alkyl (e.g., methyl or ethyl), halogen (e.g.,         Cl), —N(R₁₈)(R₁₉), hydroxy or C₁₋₆alkoxy;

    -   (viii) n=0 or 1;

    -   (ix) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R_(14,) are,         independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally         hetero)arylC₁₋₄alkoxy, (optionally hetero)arylC₁₋₄alkyl or R₁₄         can form a bridge with R₂ or R₄;

    -   (x) R₁₅ is C₁₋₄alkyl, haloC₁₋₄alkyl, —OH or —OC₁₋₄alkyl (e.g.,         —OCH₃)

    -   (xi) R₁₆ and R₁₇ are independently H or C₁₋₄alkyl;

    -   (xii) R₁₈ and R₁₉ are independently         -   H,         -   C₁₋₄alky (e.g., methyl, ethyl, n-propyl, isobutyl),         -   C₃₋₈cycloalky (e.g., cyclohexyl or cyclopenyl),         -   heteroC₃₋₈cycloalky (e.g., pyrrolidinyl, piperidinyl,             morpholinyl),         -   aryl (e.g., phenyl) or         -   heteroaryl (e.g., pyridyl),         -   wherein said aryl and heteroaryl are optionally substituted             with one or more             -   halo (e.g., fluorophenyl, e.g., 4-fluorophenyl),             -   hydroxy (e.g., hydroxyphenyl, e.g., 4-hydroxyphenyl or                 2-hydroxyphenyl),             -   C₁₋₄alkyl (e.g., methyl),             -   haloC₁₋₄alkyl (e.g., trifluoromethyl),             -   C₁₋₄carboxy, or             -   an additional aryl, heteroaryl (e.g., biphenyl or                 pyridylphenyl) or C₃₋₈cycloalkyl,

    -   (xiii) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl;         in free or salt form.

In another aspect, the invention provides a Compound of Formula I, e.g. Formula I-A and I-B:

wherein

-   -   (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂;     -   (ii) L is a single bond, —N(H)—, —CH₂—, —S—, —S(O)— or —S(O₂)—;     -   (iii) R₁ is H or C₁₋₄ alkyl (e.g., methyl);     -   (iv) R₄ is H or C₁₋₆ alkyl (e.g., methyl or isopropyl) and R₂         and R₃ are, independently,         -   H or C₁₋₆alkyl (e.g., methyl, isopropyl) optionally             substituted with halo or hydroxy (e.g., R₂ and R₃ are both             methyl, or R₂ is H and R₃ is methyl, ethyl, isopropyl or             hydroxyethyl),         -   aryl,         -   heteroaryl,         -   (optionally hetero)arylalkoxy, or         -   (optionally hetero)arylC₁₋₆alkyl;         -   or         -   R₂ is H and R₃ and R₄ together form a di-, tri- or             tetramethylene bridge (pref. wherein the R₃ and R₄ together             have the cis configuration, e.g., where the carbons carrying             R₃ and R₄ have the R and S configurations, respectively);     -   (v) R₅ is         -   a) -D-E-F, wherein:             -   D is C₁₋₄alkylene (e.g., methylene, ethylene or                 prop-2-yn-1-ylene);             -   E is a single bond, C₂₋₄alkynylene (e.g., —C≡C—),                 arylene (e.g., phenylene) or heteroarylene (e.g.,                 pyridylene);             -   F is                 -   H,                 -   aryl (e.g., phenyl),                 -   heteroaryl (e.g., pyridyl, diazolyl, triazolyl, for                     example, pyrid-2-yl, imidazol-1-yl,                     1,2,4-triazol-1-yl),                 -   halo (e.g., F, Br, Cl),                 -   haloC₁₋₄alkyl (e.g., trifluoromethyl),                 -   —C(O)—R₁₅,                 -   —N(R₁₆)(R₁₇), or                 -   C₃₋₇cycloalkyl optionally containing at least one                     atom selected from a group consisting of N or O                     (e.g., cyclopentyl, cyclohexyl, pyrrolidinyl (e.g.,                     pyrrolidin-3-yl), tetrahydro-2H-pyran-4-yl, or                     morpholinyl);             -   wherein D, E and F are independently and optionally                 substituted with one or more halo (e.g., F, Cl or Br),                 C₁₋₄alkyl (e.g., methyl), haloC₁₋₄alkyl (e.g.,                 trifluoromethyl), for example, F is heteroaryl, e.g.,                 pyridyl substituted with one or more halo (e.g.,                 6-fluoropyrid-2-yl, 5-fluoropyrid-2-yl,                 6-fluoropyrid-2-yl, 3-fluoropyrid-2-yl,                 4-fluoropyrid-2-yl, 4,6-dichloropyrid-2-yl),                 haloC₁₋₄alkyl (e.g., 5-trifluoromethylpyrid-2-yl) or                 C₁₋₄alkyl (e.g., 5-methylpyrid-2-yl), or F is aryl,                 e.g., phenyl, substituted with one or more halo (e.g.,                 4-fluorophenyl) or F is a C₃₋₇heterocycloalkyl (e.g.,                 pyrrolidinyl) optionally substituted with a C₁₋₆alkyl                 (e.g., 1-methylpyrrolidin-3-yl); or         -   b) a substituted heteroarylalkyl, e.g., substituted with             haloalkyl;         -   c) attached to the nitrogen on the pyrrolo portion of             Formula I-A or I-B and is a moiety of Formula A

-   -   -   wherein X, Y and Z are, independently, N or C, and R₈, R₉,             R₁₁ and R₁₂ are independently H or halogen (e.g., Cl or F),             and R₁₀ is             -   halogen,             -   C₁₋₄alkyl,             -   C₃₋₇cycloalkyl,             -   C₁₋₄haloalkyl (e.g., trifluoromethyl),             -   aryl (e.g., phenyl),             -   heteroaryl (e.g., pyridyl (for example pyrid-2-yl), or                 thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl)), diazolyl,                 triazolyl, tetrazolyl,             -   arylcarbonyl (e.g., benzoyl),             -   alkylsulfonyl (e.g., methylsulfonyl),             -   heteroarylcarbonyl, or             -   alkoxycarbonyl;         -   provided that when X, Y, or Z is nitrogen, R₈, R₉, or R₁₀,             respectively, is not present;

    -   (vi) R₆ is         -   H,         -   C₁₋₄alkyl,         -   C₃₋₇cycloalkyl (e.g., cyclopentyl),         -   aryl (e.g., phenyl),         -   heteroaryl (e.g., pyrid-4-yl),         -   arylC₁₋₄alkyl (e.g., benzyl),         -   arylamino (e.g., phenylamino),         -   heteroarylamino,         -   N,N-diC₁₋₄alkylamino,         -   N,N-diarylamino,         -   N-aryl-N-(arylC₁₋₄alkyl)amino (e.g.,             N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino), or         -   —N(R₁₈)(R₁₉);         -   wherein the aryl or heteroaryl is optionally substituted             with one or more halo (e.g., F, Cl), hydroxy or C₁₋₆alkoxy;

    -   (vii) R₇ is H, C₁₋₆alkyl, halogen (e.g., Cl), —N(R₁₈)(R₁₉);

    -   (viii) n=0 or 1;

    -   (ix) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R_(14,) are,         independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally         hetero)arylC₁₋₄alkoxy or (optionally hetero)arylC₁₋₄alkyl;

    -   (x) R₁₅ is C₁₋₄alkyl, haloC₁₋₄alkyl, —OH or —OC₁₋₄alkyl (e.g.,         —OCH₃)

    -   (xi) R₁₆ and R₁₇ are independently H or C₁₋₄alkyl;

    -   (xii) R₁₈ and R₁₉ are independently H, C₁₋₄alky or aryl (e.g.,         phenyl) wherein said aryl is optionally substituted with one or         more halo (e.g., fluorophenyl, e.g., 4-fluorophenyl) or hydroxy         (e.g., hydroxyphenyl, e.g., 4-hydroxyphenyl or 2-hydroxyphenyl)

    -   (xiii) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl;         in free, salt or prodrug form.

The invention further provides compounds of Formula I (I-A and I-B) as follows:

-   -   1.1 Formula I-A or I-B, wherein Q is C(═O), C(═S), C(═N(R₂₀)) or         CH₂;     -   1.2 Formula I-A or I-B or 1.1, wherein Q is C(═S);     -   1.3 Formula I-A or I-B or 1.1, wherein Q is C(═N(R₂₀));     -   1.4 Formula I-A or I-B or 1.1, wherein Q is CH₂;     -   1.5 Formula I-A or I-B or 1.1, wherein Q is C(═O);     -   1.6 Formula I-A or I-B, or any of 1.1-1.5, wherein L is a single         bond, —N(H)—, —CH₂—, —S—, —S(O)— or —S(O₂)—;     -   1.7 Formula 1.6, wherein L is a single bond;     -   1.8 Formula 1.6, wherein L is —N(H)—;     -   1.9 Formula 1.6, wherein L is —CH₂—;     -   1.10 Formula 1.6, wherein L is —S—;     -   1.11 Formula 1.6, wherein L is —S(O)—;     -   1.12 Formula 1.6, wherein L is —S(O₂)—;     -   1.13 Formula I-A or I-B, or any of 1.1-1.12, wherein R₁ is H or         C₁₋₄ alkyl (e.g., methyl);     -   1.14 Formula 1.13, wherein R₁ is H;     -   1.15 Formula 1.13, wherein R₁ is C₁₋₄ alkyl (e.g., methyl);     -   1.16 Formula I-A or I-B, or any of 1.1-1.15, wherein R₄ is H or         C₁₋₆ alkyl (e.g., methyl, isopropyl) and R₂ and R₃ are,         independently,         -   H or C₁₋₆alkyl optionally substituted with halo or hydroxy             (e.g., R₂ and R₃ are both methyl, or R₂ is H and R₃ is             methyl, ethyl, isopropyl or hydroxyethyl),         -   aryl,         -   heteroaryl,         -   (optionally hetero)arylalkoxy, or         -   (optionally hetero)arylC₁₋₆alkyl;     -   1.17 Formula I-A or I-B, or any of 1.1-1.15, wherein R₂ is H and         R₃ and R₄ together form a di-, tri- or tetramethylene bridge         (pref. wherein the R₃ and R₄ together have the cis         configuration, e.g., where the carbons carrying R₃ and R₄ have         the R and S configurations, respectively);     -   1.18 Formula I-A or I-B or any of 1.1-1.17, wherein R₅ is         -D-E-F;     -   1.19 Formula 1.18, wherein D is C₁₋₄alkylene (e.g., methylene,         ethylene or prop-2-yn-1-ylene);     -   1.20 Formula 1.19, wherein D is methylene;     -   1.21 Any of formulae 1.18-1.20, wherein E is a single bond,         C₂₋₄alkynylene (e.g., —C≡C—), arylene (e.g., phenylene) or         heteroarylene (e.g., pyridylene);     -   1.22 Any of formulae 1.18-1.20, wherein E is arylene (e.g.,         phenylene);     -   1.23 Any of formulae 1.18-1.20, wherein E is phenylene;     -   1.24 Any of formulae 1.18-1.20, wherein E is heteroarylene         (e.g., pyridylene);     -   1.25 Any of formulae 1.18-1.20, wherein E is phenylene wherein F         is para-substituted;     -   1.26 Any of formulae 1.18-1.20, wherein E is heteroarylene         (e.g., pyridylene);     -   1.27 Any of formulae 1.18-1.20, wherein E is a single bond;     -   1.28 Any of formulae 1.18-1.27, wherein F is H, aryl (e.g.,         phenyl), heteroaryl (e.g., pyridyl, e.g., pyrid-2-yl), halo         (e.g., F, Br, Cl), haloC₁₋₄alkyl (e.g., trifluoromethyl),         —C(O)—R₁₅, —N(R₁₆)(R₁₇), or C₃₋₄cycloalkyl optionally containing         at least one atom selected from a group consisting of N or O         (e.g., cyclopentyl, cyclohexyl, pyrrolidinyl (e.g.,         pyrrolidin-3-yl), tetrahydro-2H-pyran-4-yl, or morpholinyl);     -   1.29 Formula 1.28, wherein F is haloC₁₋₄alkyl (e.g.,         trifluoromethyl);     -   1.30 Formula 1.28, wherein F is trifluoromethyl;     -   1.31 Formula 1.28, wherein F is halo (e.g., F, Br, Cl);     -   1.32 Formula 1.28, wherein F is Cl;     -   1.33 Formula 1.28, wherein F is heteroaryl (e.g., pyridyl, e.g.,         pyrid-2-yl);     -   1.34 Formula 1.28, wherein F is pyridyl;     -   1.35 Formula 1.28, wherein F is pyrid-2-yl;     -   1.36 Formula 1.28, wherein F is C₃₋₇cycloalkyl optionally         containing at least one atom selected from a group consisting of         N or O (e.g., cyclopentyl, cyclohexyl, pyrrolidinyl (e.g.,         pyrrolidin-3-yl), tetrahydro-2H-pyran-4-yl, morpholinyl);     -   1.37 Formula 1.28, wherein F is cyclohexyl;     -   1.38 Formula 1.28, wherein F is pyrrolidinyl (e.g.,         pyrrolidin-3-yl);     -   1.39 Formula 1.28, wherein F is cyclopentyl;     -   1.40 Formula 1.28, wherein F is tetrahydro-2H-pyran-4-yl;     -   1.41 Formula 1.28, wherein F is aryl (e.g., phenyl);     -   1.42 Formula 1.28, wherein F is phenyl;     -   1.43 Formula 1.28, wherein F is 4-fluorophenyl;     -   1.44 Formula 1.28, wherein F is —C(O)—R₁₅ and R₁₅ is C₁₋₄alkyl         (e.g., methyl), haloC₁₋₄alkyl (e.g., trifluoromethyl), —OH or         —OC₁₋₄alkyl (e.g., —OCH₃);     -   1.45 Any of formulae 1.18-1.44, wherein D, E and F are         independently and optionally substituted with one or more halo         (e.g., F, Cl or Br), C₁₋₄alkyl (e.g., methyl), haloC₁₋₄alkyl         (e.g., trifluoromethyl), for example, F is heteroaryl, e.g.,         pyridyl substituted with one or more halo (e.g.,         6-fluoropyrid-2-yl, 5-fluoropyrid-2-yl, 6-fluoropyrid-2-yl,         3-fluoropyrid-2-yl, 4-fluoropyrid-2-yl, 4,6-dichloropyrid-2-yl),         haloC₁₋₄alkyl (e.g., 5-trifluoromethylpyrid-2-yl) or C₁₋₄alkyl         (e.g., 5-methylpyrid-2-yl), or F is aryl, e.g., phenyl,         substituted with one or more halo (e.g., 4-fluorophenyl), or F         is a C₃₋₇heterocycloalkyl (e.g., pyrrolidinyl) optionally         substituted with a C₁₋₆alkyl (e.g., 1-methylpyrrolidin-3-yl);     -   1.46 Formula 1.45, wherein F is substituted with one or more         halo (e.g., F, Cl or Br), C₁₋₄alkyl (e.g., methyl),         haloC₁₋₄alkyl (e.g., trifluoromethyl);     -   1.47 Formula 1.45, wherein F is 6-fluoropyrid-2-yl;     -   1.48 Formula 1.45, wherein F is 3-fluoropyrid-2-yl;     -   1.49 Formula 1.45, wherein F is 4-fluoropyrid-2-yl;     -   1.50 Formula 1.45, wherein F is 5-fluoropyrid-2-yl;     -   1.51 Formula 1.45, wherein F is heteroaryl, e.g., pyridyl,         optionally substituted with one or more haloC₁₋₄alkyl (e.g.,         5-trifluoromethylpyrid-2-yl;     -   1.52 Formula 1.45, wherein F is 5-trifluoromethylpyrid-2-yl;     -   1.53 Formula 1.45, wherein F is heteroaryl, e.g., pyridyl,         optionally substituted with one or more C₁₋₄alkyl (e.g.,         5-methylpyrid-2-yl);     -   1.54 Formula 1.45, wherein F is 5-methylpyrid-2-yl;     -   1.55 Formula 1.28, wherein F is —C(O)—R₁₅ and R₁₅ is methyl;     -   1.56 Formula 1.28, wherein F is —C(O)—R₁₅ and R₁₅ is         trifluoromethyl;     -   1.57 Formula 1.28, wherein F is —C(O)—R₁₅ and R₁₅ is —OH;     -   1.58 Formula 1.28, wherein F is —C(O)—R₁₅ and R₁₅ is —OC₁₋₄alkyl         (e.g., —OCH₃);     -   1.59 Formula 1.28, wherein F is —C(O)—R₁₅ and R₁₅ is —OCH₃;     -   1.60 Formula 1.28, wherein F is —N(R₁₆)(R₁₇);     -   1.61 Formula I-A or I-B or any of 1.1-1.17, wherein R₅ is a         substituted heteroarylalkyl, e.g., substituted with haloalkyl;     -   1.62 Formula I-A or I-B or any of 1.1-1.17, wherein R₅ is         attached to one of the nitrogens on the pyrazolo portion of         Formula I-A or I-B and is a moiety of Formula A

-   -   -   wherein X, Y and Z are, independently, N or C, and R₈, R₉,             R₁₁ and R₁₂ are independently H or halogen (e.g., Cl or F),             and R₁₀ is halogen, C₁₋₄alkyl, C₃₋₇cycloalkyl, C₁₋₄haloalkyl             (e.g., trifluoromethyl), aryl (e.g., phenyl), heteroaryl             (e.g., pyridyl (for example pyrid-2-yl), or thiadiazolyl             (e.g., 1,2,3-thiadiazol-4-yl)), diazolyl, triazolyl,             tetrazolyl, arylcarbonyl (e.g., benzoyl), alkylsulfonyl             (e.g., methylsulfonyl), heteroarylcarbonyl, or             alkoxycarbonyl; provided that when X, Y, or Z is nitrogen,             R₈, R₉, or R₁₀, respectively, is not present

    -   1.63 Formula 1.62, wherein R₅ is a substituted heteroarylmethyl,         e.g., para-substituted with haloalkyl;

    -   1.64 Formula 1.62, wherein R₅ is a moiety of Formula A wherein         R₈, R₉, R₁₁, and R₁₂ are H and R₁₀ is phenyl;

    -   1.65 Formula 1.62, wherein R₅ is a moiety of Formula A wherein         R₈, R₉, R₁₁, and R₁₂ are H and R₁₀ is pyridyl or thiadiazolyl;

    -   1.66 Formula 1.62, wherein R₅ is a moiety of Formula A wherein         R₈, R₉, R₁₁, and R₁₂ are, independently, H or halogen, and R₁₀         is haloalkyl;

    -   1.67 Formula 1.62, wherein R₅ is a moiety of Formula A wherein         R₈, R₉, R₁₁, and R₁₂ are, independently, H, and R₁₀ is alkyl         sulfonyl;

    -   1.68 Formula I-A or I-B or any of 1.1-1.67, wherein R₆ is H,         C₁₋₄alkyl, C₃₋₇cycloalkyl (e.g., cyclopentyl), aryl, heteroaryl,         arylC₁₋₄alkyl (e.g., benzyl), arylamino (e.g., phenylamino),         heteroarylamino, N,N-diC₁₋₄alkylamino, N,N-diarylamino,         N-aryl-N-(arylC₁₋₄alkyl)amino (e.g.,         N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino), or —N(R₁₈)(R₁₉),         wherein the aryl or heteroaryl is optionally substituted with         one or more halo (e.g., F, Cl), hydroxy or C₁₋₆alkoxy;

    -   1.69 Formula 1.68, wherein R₆ is H;

    -   1.70 Formula 1.68, wherein R₆ is aryl (e.g., phenyl) optionally         substituted with one or more halo (e.g., F, Cl), hydroxy or         C₁₋₆alkoxy;

    -   1.71 Formula 1.68, wherein R₆ is C₁₋₄alkyl;

    -   1.72 Formula 1.68, wherein R₆ is C₃₋₇cycloalkyl (e.g.,         cyclopentyl);

    -   1.73 Formula 1.68, wherein R₆ is fluorophenyl (e.g.,         4-fluorophenyl) or hydroxyphenyl (e.g., 4-hydroxyphenyl or         2-hydroxyphenyl);

    -   1.74 Formula I-A or I-B or any of 1.1-1.73, wherein R₇ is H,         C₁₋₆alkyl (e.g., methyl), halogen, —N(R₁₈)(R₁₉);

    -   1.75 Formula 1.74, wherein R₇ is H;

    -   1.76 Formula 1.74, wherein R₇ is C₁₋₆alkyl (e.g., methyl);

    -   1.77 Formula 1.74, wherein R₇ is methyl;

    -   1.78 Formula 1.74, wherein R₇ is ethyl;

    -   1.79 Formula I-A or I-B or any of 1.1-1.78, wherein n=0;

    -   1.80 Formula I-A or I-B or any of 1.1-1.78, wherein n=1;

    -   1.81 Formula 1.80, wherein n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃         and R_(14,) are, independently, H or C₁₋₄alkyl, aryl,         heteroaryl, (optionally hetero)arylC₁₋₄alkoxy or (optionally         hetero)arylC₁₋₄alkyl;

    -   1.82 any of the preceding formulae wherein the compound is         Formula I-A;

    -   1.83 any of the preceding formulae wherein the compound is         selected from a group consisting of:

-   -   1.84 any of the preceding formulae wherein the compounds inhibit         phosphodiesterase-mediated (e.g., PDE1-mediated, especially         PDE1B-mediated) hydrolysis of cGMP, e.g., with an IC₅₀ of less         than 1 μM, preferably less than 750 nM, more preferably less         than 500 nM, more preferably less than 50 nM in an         immobilized-metal affinity particle reagent PDE assay, for         example, as described in Example 16,         in free or salt form.

In still another embodiment, the invention provides a compound as follows:

-   -   2.1 a Compound of Formula I-A, I-B, II-A or II-B, or any of         1.1-1.6, 1.14-1.67, 1.74-1.84, wherein Lisa single bond or         —CH₂—;     -   2.2 formula 2.1, wherein R₆ is         -   H,         -   arylamino (e.g., phenylamino),         -   heteroarylamino,         -   N,N-diC₁₋₄alkylamino,         -   N,N-diarylamino,         -   N-aryl-N-(arylC₁₋₄alkyl)amino (e.g.,             N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino), or         -   —N(R₁₈)(R₁₉),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;     -   2.3 a Compound of Formula I-A, I-B, II-A or II-B, or any of         1.1-1.6, 1.14-1.67, 1.74-1.84, wherein L is a single bond,         —CH₂—, —N(H)—, —S—, —S(O)— or —S(O₂)—;     -   2.4 a formula 2.3, wherein R₆ is         -   H,         -   C₁₋₄alkyl (e.g., methyl, ethyl, n-propyl, isobutyl),         -   C₃₋₇cycloalkyl (e.g., cyclopentyl or cyclohexyl),         -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl, piperidinyl,             morpholinyl),         -   aryl (e.g., phenyl),         -   heteroaryl (e.g., pyrid-4-yl),         -   arylC₁₋₄alkyl (e.g., benzyl),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;     -   2.5 a Compound of Formula I-A, I-B, II-A or II-B, or any of         2.1[0010]-2.4, wherein R₅ is attached to the nitrogen on the         pyrrolo portion of Formula I-A, I-B, II-A or II-B and is a         moiety of Formula A

-   -   -   wherein X, Y and Z are, independently, N or C, and R₈, R₉,             R₁₁ and R₁₂ are independently H or halogen (e.g., Cl or F),             and R₁₀ is             -   C₃₋₇cycloalkyl,             -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl or                 piperidinyl),             -   aryl (e.g., phenyl), or             -   heteroaryl (e.g., pyridyl (for example pyrid-2-yl or                 pyrid-4-yl), or thiadiazolyl (e.g.,                 1,2,3-thiadiazol-4-yl)), diazolyl (e.g., imidazol-1-yl),                 triazolyl (e.g., 1,2,4-triazol-1-yl), tetrazolyl,             -   wherein the aryl, heteroaryl, cycloalkyl or                 heterocycloalkyl is independently, optionally                 substituted with one or more C₁₋₄alkyl (e.g., methyl),                 halogen (e.g., chloro or fluoro), haloC₁₋₄alkyl (e.g.,                 trifluoromethyl), hydroxy, C₁₋₄carboxy, —SH or an                 additional aryl or heteroaryl (e.g., biphenyl or                 pyridylphenyl),             -   provided that when X, Y, or Z is nitrogen, R₈, R₉, or                 R₁₀, respectively, is not present;

    -   2.6 Formula I-A, I-B, II-A or II-B or any of 2.1-2.5, wherein         n=0;

    -   2.7 Formula I-A, I-B, II-A or II-B or any of 2.1-2.5, wherein         n=1;

    -   2.8 Any of the preceding formulae wherein L is —N(H)—, —S—,         —S(O)— or —S(O₂)— and R₆ is:         -   H,         -   C₁₋₄alkyl (e.g., methyl, ethyl, n-propyl, isobutyl),         -   C₃₋₇cycloalkyl (e.g., cyclopentyl or cyclohexyl),         -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl, piperidinyl,             morpholinyl),         -   aryl (e.g., phenyl),         -   heteroaryl (e.g., pyrid-4-yl),         -   arylC₁₋₄alkyl (e.g., benzyl),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;

    -   2.9 a Compound of Formula I-A, I-B, II-A or II-B, or any of the         preceding formulae, wherein the remaining substituents are as         defined in any of formula 1.1-1.84;

    -   2.10 any of the preceding formulae, wherein the compound is         selected from any of the following:

-   -   2.11 any of the preceding formulae, wherein the compound is         selected from any of the following

-   -   2.12 any of the preceding formulae, wherein the compound is         selected from any of the following:

-   -   2.13 any of the preceding formulae, wherein the compounds         inhibit phosphodiesterase-mediated (e.g., PDE1-mediated,         especially PDE1A- and/or PDE1B-mediated) hydrolysis of cGMP,         e.g., with an IC₅₀ of less than 10 μM, preferably less than 1         μM, still preferably less than 750 nM, more preferably less than         500 nM, more preferably less than 50 nM especially less than 10         nM in an immobilized-metal affinity particle reagent PDE assay,         for example, as described in Example 16,         in free or salt form.

In one embodiment, the Compound of the Invention is a Compound of Formula I-A, I-B, II-A or II-B, wherein:

-   -   (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂;     -   (ii) L is a single bond, —CH₂—, —N(H)—, —S—, —S(O)— or —S(O₂)—;     -   (iii) R₁ is H or C₁₋₄ alkyl (e.g., methyl);     -   (iv) R₄ is H or C₁₋₆ alkyl (e.g., methyl or isopropyl) and R₂         and R₃ are, independently,         -   H         -   C₁₋₆alkyl (e.g., methyl, isopropyl) optionally substituted             with halo or hydroxy (e.g., R₂ and R₃ are both methyl, or R₂             is H and R₃ is methyl, ethyl, isopropyl or hydroxyethyl),         -   aryl,         -   heteroaryl,         -   (optionally hetero)arylalkoxy,         -   (optionally hetero)arylC₁₋₆alkyl, or         -   R₂ and R₃ together form a 3-6-membered ring;         -   or         -   R₂ is H and R₃ and R₄ together form a di-, tri- or             tetramethylene bridge (pref. wherein the R₃ and R₄ together             have the cis configuration, e.g., where the carbons carrying             R₃ and R₄ have the R and S configurations, respectively);     -   (v) R₅ is         -   a) -D-E-F, wherein:             -   D is C₁₋₄alkylene (e.g., methylene, ethylene or                 prop-2-yn-1-ylene);             -   E is a single bond, C₂₋₄alkynylene (e.g., —C≡C—),                 arylene (e.g., phenylene) or heteroarylene (e.g.,                 pyridylene);             -   F is                 -   H,                 -   aryl (e.g., phenyl),                 -   heteroaryl (e.g., pyridyl, diazolyl, triazolyl, for                     example, pyrid-2-yl, imidazol-1-yl,                     1,2,4-triazol-1-yl),                 -   halo (e.g., F, Br, Cl),                 -   haloC₁₋₄alkyl (e.g., trifluoromethyl),                 -   —C(O)—R₁₅,                 -   —N(R₁₆)(R₁₇), or                 -   C₃₋₇cycloalkyl optionally containing at least one                     atom selected from a group consisting of N or O                     (e.g., cyclopentyl, cyclohexyl, pyrrolidinyl (e.g.,                     pyrrolidin-3-yl), tetrahydro-2H-pyran-4-yl, or                     morpholinyl);             -   wherein D, E and F are independently and optionally                 substituted with one or more halo (e.g., F, Cl or Br),                 C₁₋₄alkyl (e.g., methyl), haloC₁₋₄alkyl (e.g.,                 trifluoromethyl), C₁₋₄alkoxy (e.g., methoxy), hydroxy,                 C₁₋₄carboxy, or an additional aryl or heteroaryl (e.g.,                 biphenyl or pyridylphenyl),             -   for example, F is heteroaryl, e.g., pyridyl substituted                 with one or more halo (e.g., 6-fluoropyrid-2-yl,                 5-fluoropyrid-2-yl, 6-fluoropyrid-2-yl,                 3-fluoropyrid-2-yl, 4-fluoropyrid-2-yl,                 4,6-dichloropyrid-2-yl), haloC₁₋₄alkyl (e.g.,                 5-trifluoromethylpyrid-2-yl) or C₁₋₄alkyl (e.g.,                 5-methylpyrid-2-yl), or F is aryl, e.g., phenyl,                 substituted with one or more halo (e.g., 4-fluorophenyl)                 or F is a C₃₋₇heterocycloalkyl (e.g., pyrrolidinyl)                 optionally substituted with a C₁₋₆alkyl (e.g.,                 1-methylpyrrolidin-3-yl); or         -   b) a substituted heteroarylalkyl, e.g., substituted with             haloC₁₋₄alkyl;         -   c) attached to the nitrogen on the pyrrolo portion of             Formula I-A, I-B, II-A or II-B and is a moiety of Formula A

-   -   -   wherein X, Y and Z are, independently, N or C, and R₈, R₉,             R₁₁ and R₁₂ are independently H or halogen (e.g., Cl or F),             and R₁₀ is             -   halogen,             -   C₁₋₄alkyl,             -   haloC₁₋₄alkyl (e.g., triflouromethyl)             -   C₁₋₄alkoxy (e.g. methoxy),             -   C₃₋₇cycloalkyl,             -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl or                 piperidinyl),             -   hetero             -   C₁₋₄haloalkyl (e.g., trifluoromethyl),             -   aryl (e.g., phenyl),             -   heteroaryl (e.g., pyridyl (for example pyrid-2-yl or                 pyrid-4-yl), or thiadiazolyl (e.g.,                 1,2,3-thiadiazol-4-yl)), diazolyl (e.g., imidazol-1-yl),                 triazolyl (e.g., 1,2,4-triazol-1-yl), tetrazolyl,             -   arylcarbonyl (e.g., benzoyl),             -   alkylsulfonyl (e.g., methylsulfonyl),             -   heteroarylcarbonyl, or             -   alkoxycarbonyl;         -   wherein the aryl, heteroaryl, cycloalkyl or heterocycloalkyl             is independently, optionally substituted with one or more             C₁₋₄alkyl (e.g., methyl), halogen (e.g., chloro or fluoro),             haloC₁₋₄alkyl (e.g., trifluoromethyl), hydroxy, C₁₋₄carboxy,             —SH or an additional aryl or heteroaryl (e.g., biphenyl or             pyridylphenyl),         -   provided that when X, Y, or Z is nitrogen, R₈, R₉, or R₁₀,             respectively, is not present;

    -   (vi) R₆ is         -   H,         -   C₁₋₄alkyl (e.g., methyl, ethyl, n-propyl, isobutyl),         -   C₃₋₇cycloalkyl (e.g., cyclopentyl or cyclohexyl),         -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl, piperidinyl,             morpholinyl),         -   aryl (e.g., phenyl),         -   heteroaryl (e.g., pyrid-4-yl),         -   arylC₁₋₄alkyl (e.g., benzyl),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;         -   when L is a single bond, —CH₂—, —N(H)—, —S—, —S(O)— or             S(O₂)—,         -   or         -   R₆ is         -   H,         -   arylamino (e.g., phenylamino),         -   heteroarylamino,         -   N,N-diC₁₋₄alkylamino,         -   N,N-diarylamino,         -   N-aryl-N-(arylC₁₋₄alkyl)amino (e.g.,             N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino), or         -   —N(R₁₈)(R₁₉),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;         -   when L is a single bond or —CH₂—;

    -   (vii) R₇ is H, C₁₋₆alkyl (e.g., methyl or ethyl), halogen (e.g.,         Cl), —N(R₁₈)(R₁₉), hydroxy or C₁₋₆alkoxy;

    -   (viii) n=0 or 1;

    -   (ix) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R₁₄, are,         independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally         hetero)arylC₁₋₄alkoxy, (optionally hetero)arylC₁₋₄alkyl or R₁₄         can form a bridge with R₂ or R₄;

    -   (x) R₁₅ is —OH or —OC₁₋₄alkyl (e.g., —OCH₃);

    -   (xi) R₁₆ and R₁₇ are independently H or C₁₋₄alkyl;

    -   (xii) R₁₈ and R₁₉ are independently H, C₁₋₄alky (e.g., methyl,         ethyl, n-propyl, isobutyl), C₃₋₈cycloalky (e.g., cyclohexyl or         cyclopenyl), heteroC₃₋₈cycloalky (e.g., pyrrolidinyl,         piperidinyl, morpholinyl), aryl (e.g., phenyl) or heteroaryl,         wherein said aryl and heteroaryl are optionally substituted with         one or more halo (e.g., fluorophenyl, e.g., 4-fluorophenyl),         hydroxy (e.g., hydroxyphenyl, e.g., 4-hydroxyphenyl or         2-hydroxyphenyl) C₁₋₄alkyl (e.g., methyl), haloC₁₋₄alkyl (e.g.,         trifluoromethyl), C₁₋₄carboxy, or an additional aryl, heteroaryl         (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;

    -   (xiii) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl;         in free or salt form.

In still another embodiment, the Compound of the Invention is a Compound of Formula I-A, I-B, II-A or II-B, wherein:

-   -   (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂;     -   (ii) L is a single bond, —CH₂—, —N(H)—, —S—, —S(O)— or —S(O₂)—;     -   (iii) R₁ is H or C₁₋₄ alkyl (e.g., methyl);     -   (iv) R₄ is H or C₁₋₆ alkyl (e.g., methyl or isopropyl) and R₂         and R₃ are, independently,         -   H         -   C₁₋₆alkyl (e.g., methyl, isopropyl) optionally substituted             with halo or hydroxy (e.g., R₂ and R₃ are both methyl, or R₂             is H and R₃ is methyl, ethyl, isopropyl or hydroxyethyl),         -   aryl,         -   heteroaryl,         -   (optionally hetero)arylalkoxy,         -   (optionally hetero)arylC₁₋₆alkyl, or         -   R₂ and R₃ together form a 3- to 6-membered ring;         -   or         -   R₂ is H and R₃ and R₄ together form a di-, tri- or             tetramethylene bridge (pref. wherein the R₃ and R₄ together             have the cis configuration, e.g., where the carbons carrying             R₃ and R₄ have the R and S configurations, respectively);     -   (v) R₅ is attached to the nitrogen on the pyrrolo portion of         Formula I-A, I-B, II-A or II-B and is a moiety of Formula A

-   -   -   wherein X, Y and Z are, independently, N or C, and R₈, R₉,             R₁₁ and R₁₂ are independently H or halogen (e.g., Cl or F),             and R₁₀ is halogen,             -   C₁₋₄alkyl,             -   haloC₁₋₄alkyl (e.g., triflouromethyl)             -   C₁₋₄alkoxy (e.g. methoxy),             -   C₃₋₇cycloalkyl,             -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl or                 piperidinyl),             -   C₁₋₄haloalkyl (e.g., trifluoromethyl),             -   aryl (e.g., phenyl),             -   heteroaryl (e.g., pyridyl (for example pyrid-2-yl or                 pyrid-4-yl), or thiadiazolyl (e.g.,                 1,2,3-thiadiazol-4-yl)), diazolyl (e.g., imidazol-1-yl),                 triazolyl (e.g., 1,2,4-triazol-1-yl), tetrazolyl,             -   arylcarbonyl (e.g., benzoyl),             -   alkylsulfonyl (e.g., methylsulfonyl),             -   heteroarylcarbonyl, or             -   alkoxycarbonyl;             -   wherein the aryl, heteroaryl, cycloalkyl or                 heterocycloalkyl is independently, optionally                 substituted with one or more C₁₋₄alkyl (e.g., methyl),                 halogen (e.g., chloro or fluoro), haloC₁₋₄alkyl (e.g.,                 trifluoromethyl), hydroxy, C₁₋₄carboxy, —SH or an                 additional aryl or heteroaryl (e.g., biphenyl or                 pyridylphenyl),             -   provided that when X, Y, or Z is nitrogen, R₈, R₉, or                 R₁₀, respectively, is not present;

    -   (vi) R₆ is         -   H,         -   C₁₋₄alkyl (e.g., methyl, ethyl, n-propyl, isobutyl),         -   C₃₋₇cycloalkyl (e.g., cyclopentyl or cyclohexyl),         -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl, piperidinyl,             morpholinyl),         -   aryl (e.g., phenyl),         -   heteroaryl (e.g., pyrid-4-yl),         -   arylC₁₋₄alkyl (e.g., benzyl),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;         -   when L is a single bond, —CH₂—, —N(H)—, —S—, —S(O)— or             S(O₂)—,         -   or         -   R₆ is         -   H,         -   arylamino (e.g., phenylamino),         -   heteroarylamino,         -   N,N-diC₁₋₄alkylamino,         -   N,N-diarylamino,         -   N-aryl-N-(arylC₁₋₄alkyl)amino (e.g.,             N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino), or         -   —N(R₁₈)(R₁₉),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;         -   when L is a single bond or —CH₂—;

    -   (vii) R₇ is H, C₁₋₆alkyl (e.g., methyl or ethyl), halogen (e.g.,         Cl), —N(R₁₈)(R₁₉), hydroxy or C₁₋₆alkoxy;

    -   (viii) n=0 or 1;

    -   (ix) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R_(14,) are,         independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally         hetero)arylC₁₋₄alkoxy, (optionally hetero)arylC₁₋₄alkyl or R₁₄         can form a bridge with R₂ or R₄;

    -   (x) R₁₈ and R₁₉ are independently H, C₁₋₄alkyl (e.g., methyl,         ethyl, n-propyl, isobutyl), C₃₋₈cycloalky (e.g., cyclohexyl or         cyclopenyl), heteroC₃₋₈cycloalky (e.g., pyrrolidinyl,         piperidinyl, morpholinyl), aryl (e.g., phenyl) or heteroaryl,         wherein said aryl and heteroaryl are optionally substituted with         one or more halo (e.g., fluorophenyl, e.g., 4-fluorophenyl),         hydroxy (e.g., hydroxyphenyl, e.g., 4-hydroxyphenyl or         2-hydroxyphenyl) C₁₋₄alkyl (e.g., methyl), haloC₁₋₄alkyl (e.g.,         trifluoromethyl), C₁₋₄carboxy, or an additional aryl, heteroaryl         (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;

    -   (xi) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl;         in free or salt form.

In yet another embodiment, the Compound of the Invention is a Compound of Formula I-A, I-B, II-A or II-B, wherein:

-   -   (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂;     -   (ii) L is —N(H)—, —S—, —S(O)— or —S(O₂)—;     -   (iii) R₁ is H or C₁₋₄ alkyl (e.g., methyl);     -   (iv) R₄ is H or C₁₋₆ alkyl (e.g., methyl or isopropyl) and R₂         and R₃ are, independently,         -   H         -   C₁₋₆alkyl (e.g., methyl, isopropyl) optionally substituted             with halo or hydroxy (e.g., R₂ and R₃ are both methyl, or R₂             is H and R₃ is methyl, ethyl, isopropyl or hydroxyethyl),         -   aryl,         -   heteroaryl,         -   (optionally hetero)arylalkoxy,         -   (optionally hetero)arylC₁₋₆alkyl, or         -   R₂ and R₃ together form a 3- to 6-membered ring;         -   or         -   R₂ is H and R₃ and R₄ together form a di-, tri- or             tetramethylene bridge (pref. wherein the R₃ and R₄ together             have the cis configuration, e.g., where the carbons carrying             R₃ and R₄ have the R and S configurations, respectively);     -   (v) R₅ is attached to the nitrogen on the pyrrolo portion of         Formula I-A, I-B, II-A or II-B and is a moiety of Formula A

-   -   -   wherein X, Y and Z are, independently, N or C, and R₈, R₉,             R₁₁ and R₁₂ are independently H or halogen (e.g., Cl or F),             and R₁₀ is             -   C₁₋₄alkoxy (e.g. methoxy),             -   C₃₋₇cycloalkyl,             -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl or                 piperidinyl),             -   aryl (e.g., phenyl),             -   heteroaryl (e.g., pyridyl (for example pyrid-2-yl or                 pyrid-4-yl), or thiadiazolyl (e.g.,                 1,2,3-thiadiazol-4-yl)), diazolyl (e.g., imidazol-1-yl),                 triazolyl (e.g., 1,2,4-triazol-1-yl), tetrazolyl,             -   wherein the aryl, heteroaryl, cycloalkyl or                 heterocycloalkyl is independently, optionally                 substituted with one or more C₁₋₄alkyl (e.g., methyl),                 halogen (e.g., chloro or fluoro), haloC₁₋₄alkyl (e.g.,                 trifluoromethyl), hydroxy, C₁₋₄carboxy, —SH or an                 additional aryl or heteroaryl (e.g., biphenyl or                 pyridylphenyl),             -   provided that when X, Y, or Z is nitrogen, R₈, R₉, or                 R₁₀, respectively, is not present;

    -   (vi) R₆ is         -   H,         -   C₁₋₄alkyl (e.g., methyl, ethyl, n-propyl, isobutyl),         -   C₃₋₇cycloalkyl (e.g., cyclopentyl or cyclohexyl),         -   heteroC₃₋₇cycloalkyl (e.g., pyrrolidinyl, piperidinyl,             morpholinyl),         -   aryl (e.g., phenyl),         -   heteroaryl (e.g., pyrid-4-yl),         -   arylC₁₋₄alkyl (e.g., benzyl),         -   wherein the aryl and heteroaryl are optionally substituted             with one or more C₁₋₄alkyl (e.g., methyl), halogen (e.g.,             chloro or fluoro), haloC₁₋₄alkyl (e.g., trifluoromethyl),             hydroxy, C₁₋₄carboxy, or an additional aryl, heteroaryl             (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;

    -   (vii) R₇ is H, C₁₋₆alkyl (e.g., methyl or ethyl), halogen (e.g.,         Cl), —N(R₁₈)(R₁₉), hydroxy or C₁₋₆alkoxy;

    -   (viii) n=0 or 1;

    -   (ix) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R_(14,) are,         independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally         hetero)arylC₁₋₄alkoxy, (optionally hetero)arylC₁₋₄alkyl or R₁₄         can form a bridge with R₂ or R₄;

    -   (x) R₁₈ and R₁₉ are independently H, C₁₋₄alky (e.g., methyl,         ethyl, n-propyl, isobutyl), C₃₋₈cycloalky (e.g., cyclohexyl or         cyclopenyl), heteroC₃₋₈cycloalky (e.g., pyrrolidinyl,         piperidinyl, morpholinyl), aryl (e.g., phenyl) or heteroaryl,         wherein said aryl and heteroaryl are optionally substituted with         one or more halo (e.g., fluorophenyl, e.g., 4-fluorophenyl),         hydroxy (e.g., hydroxyphenyl, e.g., 4-hydroxyphenyl or         2-hydroxyphenyl) C₁₋₄alkyl (e.g., methyl), haloC₁₋₄alkyl (e.g.,         trifluoromethyl), C₁₋₄carboxy, or an additional aryl, heteroaryl         (e.g., biphenyl or pyridylphenyl) or C₃₋₈cycloalkyl;

    -   (xi) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl;         in free or salt form.

If not otherwise specified or clear from context, the following terms herein have the following meanings:

-   -   (a) “Alkyl” as used herein is a saturated or unsaturated         hydrocarbon moiety, preferably saturated, preferably having one         to six carbon atoms, which may be linear or branched, and may be         optionally mono-, di- or tri-substituted, e.g., with halogen         (e.g., chloro or fluoro), hydroxy, or carboxy.     -   (b) “Cycloalkyl” as used herein is a saturated or unsaturated         nonaromatic hydrocarbon moiety, preferably saturated, preferably         comprising three to nine carbon atoms, at least some of which         form a nonaromatic mono- or bicyclic, or bridged cyclic         structure, and which may be optionally substituted, e.g., with         halogen (e.g., chloro or fluoro), hydroxy, or carboxy. Wherein         the cycloalkyl optionally contains one or more atoms selected         from N and O and/or S, said cycloalkyl may also be a         heterocycloalkyl.     -   (c) “Heterocycloalkyl” is, unless otherwise indicated, saturated         or unsaturated nonaromatic hydrocarbon moiety, preferably         saturated, preferably comprising three to nine carbon atoms, at         least some of which form a nonaromatic mono- or bicyclic, or         bridged cyclic structure, wherein at least one carbon atom is         replaced with N, O or S, which heterocycloalkyl may be         optionally substituted, e.g., with halogen (e.g., chloro or         fluoro), hydroxy, or carboxy.     -   (d) “Aryl” as used herein is a mono or bicyclic aromatic         hydrocarbon, preferably phenyl, optionally substituted, e.g.,         with alkyl (e.g., methyl), halogen (e.g., chloro or fluoro),         haloalkyl (e.g., trifluoromethyl), hydroxy, carboxy, or an         additional aryl or heteroaryl (e.g., biphenyl or pyridylphenyl).     -   (e) “Heteroaryl” as used herein is an aromatic moiety wherein         one or more of the atoms making up the aromatic ring is sulfur         or nitrogen rather than carbon, e.g., pyridyl or thiadiazolyl,         which may be optionally substituted, e.g., with alkyl, halogen,         haloalkyl, hydroxy or carboxy.     -   (f) For ease of reference, the atoms on the pyrazolo-pyrimidine         core of the Compounds of the Invention are numbered in         accordance with the numbering depicted in Formula I, unless         otherwise noted.     -   (g) Wherein E is phenylene, the numbering is as follows:

-   -   (h) It is intended that wherein the substituents end in “ene”,         for example, alkylene, phenylene or arylalkylene, said         substitutents are intended to bridge or be connected to two         other substituents. Therefore, methylene is intended to be —CH₂—         and phenylene intended to be —C₆H₄— and arylalkylene is intended         to be —C₆H₄—CH₂— or —CH₂—C₆H₄—.     -   (i) The Compounds of the Invention are intended to be numbered         as follows:

Compounds of the Invention, e.g., substituted 4,5,7,8-tetrahydro-2H-imidazo[1,2-a]pyrrolo[3,4-e]pyrimidine or 4,5,7,8,9-pentahydro-2H-pyrimido[1,2-a]pyrrolo[3,4-e]pyrimidine, e.g., Compounds of Formula I (Formula I-A and I-B), e.g., any of formulae 1.1-1.84, or a Compound of Formula II (e.g., II-A or II-B), any of formulae 2.1-2.13 may exist in free or salt form, e.g., as acid addition salts. In this specification unless otherwise indicated, language such as “Compounds of the Invention” is to be understood as embracing the compounds in any form, for example free or acid addition salt form, or where the compounds contain acidic substituents, in base addition salt form. The Compounds of the Invention are intended for use as pharmaceuticals, therefore pharmaceutically acceptable salts are preferred. Salts which are unsuitable for pharmaceutical uses may be useful, for example, for the isolation or purification of free Compounds of the Invention or their pharmaceutically acceptable salts, are therefore also included.

Compounds of the Invention may in some cases also exist in prodrug form. A prodrug form is compound which converts in the body to a Compound of the Invention. For example when the Compounds of the Invention contain hydroxy or carboxy substituents, these substituents may form physiologically hydrolysable and acceptable esters. As used herein, “physiologically hydrolysable and acceptable ester” means esters of Compounds of the Invention which are hydrolysable under physiological conditions to yield acids (in the case of Compounds of the Invention which have hydroxy substituents) or alcohols (in the case of Compounds of the Invention which have carboxy substituents) which are themselves physiologically tolerable at doses to be administered. Therefore, wherein the Compound of the Invention contains a hydroxy group, for example, Compound-OH, the acyl ester prodrug of such compound, i.e., Compound-O—C(O)—C₁₋₄alkyl, can hydrolyze in the body to form physiologically hydrolysable alcohol (Compound-OH) on the one hand and acid on the other (e.g., HOC(O)—C₁₋₄alkyl). Alternatively, wherein the Compound of the Invention contains a carboxylic acid, for example, Compound-C(O)OH, the acid ester prodrug of such compound, Compound-C(O)O—C₁₋₄alkyl can hydrolyze to form Compound-C(O)OH and HO—C₁₋₄alkyl. As will be appreciated the term thus embraces conventional pharmaceutical prodrug forms.

The invention also provides methods of making the Compounds of the Invention and methods of using the Compounds of the Invention for treatment of diseases and disorders as set forth below (especially treatment of diseases characterized by reduced dopamine D1 receptor signaling activity, such as Parkinson's disease, Tourette's Syndrome, Autism, fragile X syndrome, ADHD, restless leg syndrome, depression, cognitive impairment of schizophrenia, narcolepsy and diseases that may be alleviated by the enhancement of progesterone-signaling such as female sexual dysfunction, or a disease or disorder such as psychosis or glaucoma). This list is not intended to be exhaustive and may include other diseases and disorders as set forth below.

In another embodiment, the invention further provides a pharmaceutical composition comprising a Compound of the Invention, in free, pharmaceutically acceptable salt or prodrug form, in admixture with a pharmaceutically acceptable carrier.

DETAILED DESCRIPTION OF THE INVENTION Methods of Making Compounds of the Invention

The compounds of the Invention and their pharmaceutically acceptable salts may be made using the methods as described and exemplified herein and by methods similar thereto and by methods known in the chemical art. Such methods include, but not limited to, those described below. If not commercially available, starting materials for these processes may be made by procedures, which are selected from the chemical art using techniques which are similar or analogous to the synthesis of known compounds. Various starting materials and/or Compounds of the Invention may be prepared using methods described in WO 2006/133261 and PCT/US2007/070551. All references cited herein are hereby incorporated by reference in their entirety.

The Compounds of the Invention include their enantiomers, diastereoisomers and racemates, as well as their polymorphs, hydrates, solvates and complexes. Some individual compounds within the scope of this invention may contain double bonds. Representations of double bonds in this invention are meant to include both the E and the Z isomer of the double bond. In addition, some compounds within the scope of this invention may contain one or more asymmetric centers. This invention includes the use of any of the optically pure stereoisomers as well as any combination of stereoisomers.

It is also intended that the Compounds of the Invention encompass their stable and unstable isotopes. Stable isotopes are nonradioactive isotopes which contain one additional neutron compared to the abundant nuclides of the same species (i.e., element). It is expected that the activity of compounds comprising such isotopes would be retained, and such compound would also have utility for measuring pharmacokinetics of the non-isotopic analogs. For example, the hydrogen atom at a certain position on the Compounds of the Invention may be replaced with deuterium (a stable isotope which is non-raradioactive). Examples of known stable isotopes include, but not limited to, deuterium, ¹³C, ¹⁵N, ¹⁸O. Alternatively, unstable isotopes, which are radioactive isotopes which contain additional neutrons compared to the abundant nuclides of the same species (i.e., element), e.g., ¹²³I, ¹³¹I, ¹²⁵I, ¹¹C, ¹⁸F, may replace the corresponding abundant species of I, C and F. Another example of useful isotope of the compound of the invention is the ¹¹C isotope. These radio isotopes are useful for radio-imaging and/or pharmacokinetic studies of the compounds of the invention.

Melting points are uncorrected and (dec) indicates decomposition. Temperature are given in degrees Celsius (° C.); unless otherwise stated, operations are carried out at room or ambient temperature, that is, at a temperature in the range of 18-25° C. Chromatography means flash chromatography on silica gel; thin layer chromatography (TLC) is carried out on silica gel plates. NMR data is in the delta values of major diagnostic protons, given in parts per million (ppm) relative to tetramethylsilane (TMS) as an internal standard. Conventional abbreviations for signal shape are used. Coupling constants (J) are given in Hz. For mass spectra (MS), the lowest mass major ion is reported for molecules where isotope splitting results in multiple mass spectral peaks Solvent mixture compositions are given as volume percentages or volume ratios. In cases where the NMR spectra are complex, only diagnostic signals are reported.

Terms and Abbreviations:

BuLi=n-butyllithium

Bu^(t)OH=tert-butyl alcohol,

CAN=ammonium cerium (IV) nitrate,

DIPEA=diisopropylethylamine,

DMF=N,N-dimethylforamide,

DMSO=dimethyl sulfoxide,

Et₂O=diethyl ether,

EtOAc=ethyl acetate,

equiv.=equivalent(s),

h=hour(s),

HPLC=high performance liquid chromatography,

LDA=lithium diisopropylamide

MeOH=methanol,

NBS=N-bromosuccinimide

NCS=N-chlorosuccinimide

NaHCO₃=sodium bicarbonate,

NH₄OH=ammonium hydroxide,

Pd₂(dba)₃=tris[dibenzylideneacetone]dipalladium(0)

PMB=p-methoxybenzyl,

POCl₃=phosphorous oxychloride,

SOCl₂=thionyl chloride,

TFA=trifluoroacetic acid,

THF=tetrahedrofuran.

The synthetic methods in this invention are illustrated below. The significances for the R groups are as set forth above for formula I unless otherwise indicated.

In an aspect of the invention, Compounds (I)-A and (I)-B may be formed by reacting a compound of 1-A and 1-B respectively with for example a R₅—X in a solvent such as DMF and a base such as K₂CO₃ at room temperature or with heating:

wherein all the substitutents are as defined previously in Formula I-A, I-B, II-A or II-B above; X is a leaving group such as a halogen, mesylate, or tosylate.

Alternatively, compounds I-A, I-B, II-A and II-B, wherein L is —N(H)—, —S—, —S(O)— or S(O)₂— may be synthesized by reacting a compound of 1-C and 1-D respectively with for example a R₆-L-H in a solvent such as DMF or in neat condition with heating:

wherein all the other substituents are as defined previously in Formula I-A, I-B, II-A or II-B above; X is a leaving group such as a halogen group.

Compound 1-C, e.g., wherein Q is C(═O) and X is a chloro group, may be prepared by, e.g., reacting compound 1-E with a chlorinating reagent such as hexachloroethane in the presence of a strong base or lithium reagent such as LiHMDS. Compound 1-D, e.g., wherein Q is C(═O) and X is a chloro group, may be prepared by, e.g., reacting compound 1-F with a chlorinating reagent such NCS (N-chlorosuccinimide) in a solvent such as CCl₄. Sometimes, when R₅ is H, a protective group such as a para-methoxybenzyl (PMB) group may be added prior to the reaction. Under this circumstance, compound 1-C or 1-D with the PMB at the pyrrolo nitrogen can be deprotected using a reagent such as TFA/TFMSA, and then reacts the resulting (deprotected pyrrolo compound) with R₅X wherein X is a leaving group such as a halogen, mesylate or tosylate, under basic conditions to yield 1-C or 1-D analogs.

Compounds (I)-E and (I)-F may be formed by reacting a compound of 1-G and 1-H respectively with for example a R₅—X in a solvent such as DMF and a base such as K₂CO₃ at room temperature or with heating:

wherein all the substituents are as defined previously in Formula I-A, I-B, II-A or II-B; X is a leaving group such as a halogen group, mesylate or tosylate.

Intermediate 2, e.g., wherein Q is C(═O) may be prepared by, e.g., reacting Intermediate 3 with sodium hydride and para-toluenesulfonylmethyl isocyanide.

Alternatively and preferably, Intermediate 2, e.g., wherein Q is C(═O) is prepared by, e.g., reacting Intermediate 3 with a strong base such as sodium hydride and a reagent such as TsCHR₇NC in a solvent such as THF:

Intermediate 3 may be prepared by, e.g., reacting Intermediate 4 with diethyl azodicarboxylate in the presence of triphenylphosphine.

Alternatively and preferably, Intermediate 3 may be prepared by, e.g., reacting Intermediate 4 with a dehydrating reagent such as diethyl azodicarboxylate in the presence of phosphine ligand such as triphenylphosphine.

Intermediate 4 may, in turn be made as similarly disclosed in WO 2006/133261, e.g., by reacting a compound of 5-A with an amino alcohol, e.g., (1R,2R)-(−)-2-hydroxycyclopentylamine hydrochloride, e.g., in the presence of, for example, DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene).

wherein all the substituents are as defined previously; X is a leaving group such as a halogen or methylthio group.

Alternatively and preferably, Intermediate 4 is prepared, e.g., by reacting a compound of 5-A with an amino alcohol in the presence of a strong base, for example, DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene),

wherein all the substituents are as defined previously; X is a leaving group such as a halogen or methylthio group.

Still alternatively, intermediate 4 may be made, e.g., by reacting a compound of 5-B with an amino alcohol in the presence of a strong base, for example, DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene) and a coupling reagent such as BOP at room temperature.

Intermediate 6 wherein X is halo, e.g., Cl, can be prepared by reacting halogenating Compound 7, e.g., reacting Compound 7 with, e.g., N-chlorosuccinimide, N-bromosuccinimide, or I₂ in the presence of, e.g., carbontetrachloride in a solvent such as DMF.

Compound 8 may be formed by reacting a compound of 9 with for example an amine such as aniline in the present of, e.g., TFA.

The thione compounds of the invention, e.g., Compounds of Formula I-A or I-B or II-A or II-B wherein Q is C(═S) may then be prepared by reacting the Compounds of the Invention wherein Q is C(═O) with P₄S₁₀ in a microwave vial in the presence of a base, e.g., pyridine, and heating the mixture to an elevated temperature, e.g., in a microwave, e.g., to about 150° C. The imine compounds of the Invention, e.g., Compounds of Formula I-A or I-B or II-A or II-B wherein Q is C(═N(R₂₀)) may in turn be converted from the thione derivative (i.e., Compounds of Formula I-A or I-B or II-A or II-B wherein with Q is C(═S) by reacting the thione derivative with NH₂(R₂₀) in the presence of HgCl₂, e.g., in a solvent such as THF, and heating the reaction mixture to an elevated temperature, e.g., in a microwave, e.g., to about 110° C.

The Compounds of the Invention, e.g., Compounds of Formula I-A or I-B or II-A or II-B wherein Q is C(R₁₄)(R₁₅) may also be prepared by reacting the ketone derivative, e.g., Formula I-A or I-B or II-A or II-B wherein Q is C(═O), with a reducing agent, e.g., diisobutylaluminum hydride (DIBAL-H), lithium aluminum hydride, sodium borohydride, preferably, DIBAL-H.

Wherein L of the compounds of the invention is —S— (thiol) or Compound (I)-C, these compounds may be prepared by reacting Compound (IVb), e.g., with phenyl disulfide and lithium bis(trimethylsilyl)azanide (LiHMDS).

wherein R₆ is phenyl.

Alternatively and preferably, wherein L of the compounds of the invention is —S— (thiol) or Compound (I)-C, these compounds may be prepared by reacting Compound 1-G, with a disulfide in the presence of a base such as lithium bis(trimethylsilyl)azanide (LiHMDS).

The sulfinyl derivatives of the Invention, e.g., Formula I wherein L is SO or SO₂ may be prepared by the oxidation of (I)-C using a oxidizing reagent such as oxone or a peroxide in a solvent such as acetonitrile and methanol.

The invention thus provides methods of making Compounds of Formula I-A, I-B or II-A or II-B, for example, comprising

-   -   (i) reacting Intermediate 1-A or 1-B with a compound of formula         R₅—X wherein X is a leaving group, e.g., halogen, mesylate, or         tosylate, R₅ is as defined above in Formula I, e.g., under basic         conditions, for example:

Methods of Using Compounds of the Invention

The Compounds of the Invention, any of the compounds disclosed herein e.g., any of Compounds of Formula I-A, I-B, e.g., any of 1.1-1.84, or Formula II-A or II-B, e.g., any of 2.1-2.13, in free or salt form are useful in the treatment of diseases characterized by disruption of or damage to cAMP and cGMP mediated pathways, e.g., as a result of increased expression of PDE1 or decreased expression of cAMP and cGMP due to inhibition or reduced levels of inducers of cyclic nucleotide synthesis, such as dopamine and nitric oxide (NO). By preventing the degradation of cAMP and cGMP by PDE1B, thereby increasing intracellular levels of cAMP and cGMP, the Compounds of the Invention potentiate the activity of cyclic nucleotide synthesis inducers.

The invention provides methods of treatment of any one or more of the following conditions:

-   -   (i) Neurodegenerative diseases, including Parkinson's disease,         restless leg, tremors, dyskinesias, Huntington's disease,         Alzheimer's disease, and drug-induced movement disorders;     -   (ii) Mental disorders, including depression, attention deficit         disorder, attention deficit hyperactivity disorder, bipolar         illness, anxiety, sleep disorders, e.g., narcolepsy, cognitive         impairment, dementia, Tourette's syndrome, autism, fragile X         syndrome, psychostimulant withdrawal, and drug addiction;     -   (iii) Circulatory and cardiovascular disorders, including         cerebrovascular disease, stroke, congestive heart disease,         hypertension, pulmonary hypertension, and sexual dysfunction;     -   (iv) Respiratory and inflammatory disorders, including asthma,         chronic obstructive pulmonary disease, and allergic rhinitis, as         well as autoimmune and inflammatory diseases;     -   (v) Any disease or condition characterized by low levels of cAMP         and/or cGMP (or inhibition of cAMP and/or cGMP signaling         pathways) in cells expressing PDE1; and/or     -   (vi) Any disease or condition characterized by reduced dopamine         D1 receptor signaling activity,         comprising administering an effective amount of a Compound of         the Invention, e.g., a compound according to any of Formula I or         1.1-1.84, in free, pharmaceutically acceptable salt or prodrug         form, to a human or animal patient in need thereof. In another         aspect, the invention provides a method of treatment of the         conditions disclosed above comprising administering a         therapeutically effective amount of a Compound of Formula II-A         or II-B, e.g., any of 2.1-2.13, in free or salt in free or         pharmaceutically acceptable salt form, or a composition         comprising the same, to a human or animal patient in need         thereof.

In an especially preferred embodiment, the invention provides methods of treatment or prophylaxis for narcolepsy. In this embodiment, PDE 1 Inhibitors may be used as a sole therapeutic agent, but may also be used in combination or for co-administration with other active agents. Thus, the invention further comprises a method of treating narcolepsy comprising administering simultaneously, sequentially, or contemporaneously administering therapeutically effective amounts of

-   -   (i) a PDE 1 Inhibitor, e.g., a compound according to any of         Formula I or any of 1.1-0, and     -   (ii) a compound to promote wakefulness or regulate sleep, e.g.,         selected from (a) central nervous system stimulants-amphetamines         and amphetamine like compounds, e.g., methylphenidate,         dextroamphetamine, methamphetamine, and pemoline; (b)         modafinil, (c) antidepressants, e.g., tricyclics (including         imipramine, desipramine, clomipramine, and protriptyline) and         selective serotonin reuptake inhibitors (including fluoxetine         and sertraline); and/or (d) gamma hydroxybutyrate (GHB).         in free or pharmaceutically acceptable salt form, to a human or         animal patient in need thereof. In still another embodiment, the         methods of treatment or prophylaxis for narcolepsy as         hereinbefore described, comprises administering a         therapeutically effective amount of a Compound of Formula II-A         or II-B, or any of Formula 2.1-2.13, in free or pharmaceutically         acceptable salt form, as a sole therapeutic agent or use in         combination for co-administered with another active agent.

In another embodiment, the invention further provides methods of treatment or prophylaxis of a condition which may be alleviated by the enhancement of the progesterone signaling comprising administering an effective amount of a Compound of the Invention, e.g., a compound according to any of Formula I, or any of 1.1-1.84, in free, pharmaceutically acceptable salt or prodrug form, to a human or animal patient in need thereof. The invention also provides methods of treatment as disclosed here, comprising administering a therapeutically effective amount of a Compound of Formula II-A or II-B, e.g., any of formulae 2.1-2.13, in free or pharmaceutically acceptable salt form. In still another embodiment, the invention further provides methods of treatment or prophylaxis of a condition which may be alleviated by the enhancement of the progesterone signaling comprising administering an effective amount of a Compound of the Invention, e.g., a compound according to any of Formula I, or any of 1.1-1.84, in free, pharmaceutically acceptable salt or prodrug form, to a human or animal patient in need thereof. In another aspect, the invention provides methods of treatment as disclosed herein, comprising administering an effective amount of a Compound of the Invention, e.g., a compound according to any of Formula II-A or II-B, e.g., e.g., any of formulae 2.1-2.13, in free or pharmaceutically acceptable salt form. Disease or condition that may be ameliorated by enhancement of progesterone signaling include, but are not limited to, female sexual dysfunction, secondary amenorrhea (e.g., exercise amenorrhoea, anovulation, menopause, menopausal symptoms, hypothyroidism), pre-menstrual syndrome, premature labor, infertility, for example infertility due to repeated miscarriage, irregular menstrual cycles, abnormal uterine bleeding, osteoporosis, autoimmmune disease, multiple sclerosis, prostate enlargement, prostate cancer, and hypothyroidism. For example, by enhancing progesterone signaling, the PDE 1 inhibitors may be used to encourage egg implantation through effects on the lining of uterus, and to help maintain pregnancy in women who are prone to miscarriage due to immune response to pregnancy or low progesterone function. The novel PDE 1 inhibitors, e.g., as described herein, may also be useful to enhance the effectiveness of hormone replacement therapy, e.g., administered in combination with estrogen/estradiol/estriol and/or progesterone/progestins in postmenopausal women, and estrogen-induced endometrial hyperplasia and carcinoma. The methods of the invention are also useful for animal breeding, for example to induce sexual receptivity and/or estrus in a nonhuman female mammal to be bred.

In this embodiment, PDE 1 Inhibitors may be used in the foregoing methods of treatment or prophylaxis as a sole therapeutic agent, but may also be used in combination or for co-administration with other active agents, for example in conjunction with hormone replacement therapy. Thus, the invention further comprises a method of treating disorders that may be ameliorated by enhancement of progesterone signaling comprising administering simultaneously, sequentially, or contemporaneously administering therapeutically effective amounts of

-   -   (i) a PDE 1 Inhibitor, e.g., a compound according to any of         Formula I-A or I-B or any of 1.1-1.84, and     -   (ii) a hormone, e.g., selected from estrogen and estrogen         analogues (e.g., estradiol, estriol, estradiol esters) and         progesterone and progesterone analogues (e.g., progestins)         in free or pharmaceutically acceptable salt form, to a human or         animal patient in need thereof. In another embodiment, the         invention provides the method described above wherein the PDE 1         inhibitor is a Compound of Formula II-A or II-B, e.g., any of         formulae 2.1-2.13, in free or pharmaceutically acceptable salt         form.

The invention also provides a method for enhancing or potentiating dopamine D1 intracellular signaling activity in a cell or tissue comprising contacting said cell or tissue with an amount of a Compound of the Invention, e.g., Formula I-A or I-B or any of 1.1-1.84, sufficient to inhibit PDE1B activity. The invention further provides a method for enhancing or potentiating dopamine D1 intracellular signaling activity in a cell or tissue comprising contacting said cell or tissue with an amount of a Compound of Formula II-A or II-B or any of 2.1-2.13, in free or salt form.

The invention also provides a method for treating a PDE1-related, especially PDE1B-related disorder, a dopamine D1 receptor intracellular signaling pathway disorder, or disorders that may be alleviated by the enhancement of the progesterone signaling pathway in a patient in need thereof comprising administering to the patient an effective amount of a Compound of the Invention, e.g., Formula I, e.g., Formula I-A or I-B or any of 1.1-1.84, that inhibits PDE1B, wherein PDE1B activity modulates phosphorylation of DARPP-32 and/or the GluR1 AMPA receptor. Similarly, the invention provides a method for treating a PDE1-related, especially PDE1B-related disorder, a dopamine D1 receptor intracellular signaling pathway disorder, or disorders that may be alleviated by the enhancement of the progesterone signaling pathway in a patient in need thereof comprising administering to the patient an effective amount of a Compound of Formula II, e.g., II-A or II-B or any of 2.1-2.13, in free or pharmaceutically acceptable salt form.

“The Compound of the Invention” referred to above includes a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form.

In another aspect, the invention also provides a method for the treatment for glaucoma or elevated intraocular pressure comprising topical administration of a therapeutically effective amount of a phospodiesterase type I (PDE1) Inhibitor of the Invention, e.g., a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form, in an opthalmically compatible carrier to the eye of a patient in need thereof. However, treatment may alternatively include a systemic therapy. Systemic therapy includes treatment that can directly reach the bloodstream, or oral methods of administration, for example.

The invention further provides a pharmaceutical composition for topical ophthalmic use comprising a PDE1 inhibitor; for example an ophthalmic solution, suspension, cream or ointment comprising a PDE1 Inhibitor of the Invention, e.g., a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or ophthamalogically acceptable salt form, in combination or association with an ophthamologically acceptable diluent or carrier.

Optionally, the PDE1 inhibitor may be administered sequentially or simultaneously with a second drug useful for treatment of glaucoma or elevated intraocular pressure. Where two active agents are administered, the therapeutically effective amount of each agent may be below the amount needed for activity as monotherapy. Accordingly, a subthreshold amount (i.e., an amount below the level necessary for efficacy as monotherapy) may be considered therapeutically effective and also may also be referred alternatively as an effective amount. Indeed, an advantage of administering different agents with different mechanisms of action and different side effect profiles may be to reduce the dosage and side effects of either or both agents, as well as to enhance or potentiate their activity as monotherapy.

The invention thus provides the method of treatment of a condition selected from glaucoma and elevated intraocular pressure comprising administering to a patient in need thereof an effective amount, e.g., a subthreshold amount, of an agent known to lower intraocular pressure concomitantly, simultaneously or sequentially with an effective amount, e.g., a subthreshold amount, of a PDE1 Inhibitor of the Invention, e.g., a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form, such that amount of the agent known to lower intraocular pressure and the amount of the PDE1 inhibitor in combination are effective to treat the condition.

In one embodiment, one or both of the agents are administered topically to the eye. Thus the invention provides a method of reducing the side effects of treatment of glaucoma or elevated intraocular pressure by administering a reduced dose of an agent known to lower intraocular pressure concomitantly, simultaneously or sequentially with an effective amount of a PDE1 inhibitor. However, methods other than topical administration, such as systemic therapeutic administration, may also be utilized.

The optional additional agent or agents for use in combination with a PDE1 inhibitor may, for example, be selected from the existing drugs comprise typically of instillation of a prostaglandin, pilocarpine, epinephrine, or topical beta-blocker treatment, e.g. with timolol, as well as systemically administered inhibitors of carbonic anhydrase, e.g. acetazolamide. Cholinesterase inhibitors such as physostigmine and echothiopate may also be employed and have an effect similar to that of pilocarpine. Drugs currently used to treat glaucoma thus include, e.g.,

-   -   1. Prostaglandin analogs such as latanoprost (Xalatan),         bimatoprost (Lumigan) and travoprost (Travatan), which increase         uveoscleral outflow of aqueous humor. Bimatoprost also increases         trabecular outflow.     -   2. Topical beta-adrenergic receptor antagonists such as timolol,         levobunolol (Betagan), and betaxolol, which decrease aqueous         humor production by the ciliary body.     -   3. Alpha₂-adrenergic agonists such as brimonidine (Alphagan),         which work by a dual mechanism, decreasing aqueous production         and increasing uveo-scleral outflow.     -   4. Less-selective sympathomimetics like epinephrine and         dipivefrin (Propine) increase outflow of aqueous humor through         trabecular meshwork and possibly through uveoscleral outflow         pathway, probably by a beta₂-agonist action.     -   5. Miotic agents (parasympathomimetics) like pilocarpine work by         contraction of the ciliary muscle, tightening the trabecular         meshwork and allowing increased outflow of the aqueous humour.     -   6. Carbonic anhydrase inhibitors like dorzolamide (Trusopt),         brinzolamide (Azopt), acetazolamide (Diamox) lower secretion of         aqueous humor by inhibiting carbonic anhydrase in the ciliary         body.     -   7. Physostigmine is also used to treat glaucoma and delayed         gastric emptying.

For example, the invention provides pharmaceutical compositions comprising a PDE1 Inhibitor of the Invention and an agent selected from (i) the prostanoids, unoprostone, latanoprost, travoprost, or bimatoprost; (ii) an alpha adrenergic agonist such as brimonidine, apraclonidine, or dipivefrin and (iii) a muscarinic agonist, such as pilocarpine. For example, the invention provides ophthalmic formulations comprising a PDE-1 Inhibitor of the Invention together with bimatoprost, abrimonidine, brimonidine, timolol, or combinations thereof, in free or ophthamalogically acceptable salt form, in combination or association with an ophthamologically acceptable diluent or carrier. In addition to selecting a combination, however, a person of ordinary skill in the art can select an appropriate selective receptor subtype agonist or antagonist. For example, for alpha adrenergic agonist, one can select an agonist selective for an alpha 1 adrenergic receptor, or an agonist selective for an alpha₂ adrenergic receptor such as brimonidine, for example. For a beta-adrenergic receptor antagonist, one can select an antagonist selective for either β₁, or β₂, or β₃, depending on the appropriate therapeutic application. One can also select a muscarinic agonist selective for a particular receptor subtype such as M₁-M₅.

The PDE 1 inhibitor may be administered in the form of an ophthalmic composition, which includes an ophthalmic solution, cream or ointment. The ophthalmic composition may additionally include an intraocular-pressure lowering agent.

In yet another example, the PDE-1 Inhibitors disclosed may be combined with a subthreshold amount of an intraocular pressure-lowering agent which may be a bimatoprost ophthalmic solution, a brimonidine tartrate ophthalmic solution, or brimonidine tartrate/timolol maleate ophthalmic solution.

In addition to the above-mentioned methods, it has also been surprisingly discovered that PDE1 inhibitors are useful to treat psychosis, for example, any conditions characterized by psychotic symptoms such as hallucinations, paranoid or bizarre delusions, or disorganized speech and thinking, e.g., schizophrenia, schizoaffective disorder, schizophreniform disorder, psychotic disorder, delusional disorder, and mania, such as in acute manic episodes and bipolar disorder. Without intending to be bound by any theory, it is believed that typical and atypical antipsychotic drugs such as clozapine primarily have their antagonistic activity at the dopamine D2 receptor. PDE1 inhibitors, however, primarily act to enhance signaling at the dopamine D1 receptor. By enhancing D1 receptor signaling, PDE1 inhibitors can increase NMDA receptor function in various brain regions, for example in nucleus accumbens neurons and in the prefrontal cortex. This enhancement of function may be seen for example in NMDA receptors containing the NR2B subunit, and may occur e.g., via activation of the Src and protein kinase A family of kinases.

Therefore, the invention provides a new method for the treatment of psychosis, e.g., schizophrenia, schizoaffective disorder, schizophreniform disorder, psychotic disorder, delusional disorder, and mania, such as in acute manic episodes and bipolar disorder, comprising administering a therapeutically effective amount of a phosphodiesterase-1 (PDE1) Inhibitor of the Invention, e.g., a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form, to a patient in need thereof.

PDE 1 Inhibitors may be used in the foregoing methods of treatment prophylaxis as a sole therapeutic agent, but may also be used in combination or for co-administration with other active agents. Thus, the invention further comprises a method of treating psychosis, e.g., schizophrenia, schizoaffective disorder, schizophreniform disorder, psychotic disorder, delusional disorder, or mania, comprising administering simultaneously, sequentially, or contemporaneously administering therapeutically effective amounts of:

-   -   (i) a PDE 1 Inhibitor of the invention, e.g., a a Compound of         Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of         Formula II-A or II-B, e.g., any of 2.1-2.13, in free or         pharmaceutically acceptable salt form; and     -   (ii) an antipsychotic, e.g.,

Typical antipsychotics, e.g.,

-   -   Butyrophenones, e.g. Haloperidol (Haldol, Serenace), Droperidol         (Droleptan);     -   Phenothiazines, e.g., Chlorpromazine (Thorazine, Largactil),         Fluphenazine (Prolixin), Perphenazine (Trilafon),         Prochlorperazine (Compazine), Thioridazine (Mellaril, Melleril),         Trifluoperazine (Stelazine), Mesoridazine, Periciazine,         Promazine, Triflupromazine (Vesprin), Levomepromazine (Nozinan),         Promethazine (Phenergan), Pimozide (Orap);     -   Thioxanthenes, e.g., Chlorprothixene, Flupenthixol (Depixol,         Fluanxol), Thiothixene (Navane), Zuclopenthixol (Clopixol,         Acuphase);

Atypical antipsychotics, e.g.,

-   -   Clozapine (Clozaril), Olanzapine (Zyprexa), Risperidone         (Risperdal), Quetiapine (Seroquel), Ziprasidone (Geodon),         Amisulpride (Solian), Paliperidone (Invega), Aripiprazole         (Abilify), Bifeprunox; norclozapine,

in free or pharmaceutically acceptable salt form, to a patient in need thereof.

In a particular embodiment, the Compounds of the Invention are particularly useful for the treatment or prophylaxis of schizophrenia.

Compounds of the Invention, e.g., a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form, are particularly useful for the treatment of Parkinson's disease, schizophrenia, narcolepsy, glaucoma and female sexual dysfunction.

In still another aspect, the invention provides a method of lengthening or enhancing growth of the eyelashes by administering an effective amount of a prostaglandin analogue, e.g., bimatoprost, concomitantly, simultaneously or sequentially with an effective amount of a PDE1 inhibitor of the Invention, e.g., a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form, to the eye of a patient in need thereof.

In yet another aspect, the invention provides a method for the treatment or prophylaxis of traumatic brain injury comprising administering a therapeutically effective amount of a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form, to a patient in need thereof Traumatic brain injury (TBI) encompasses primary injury as well as secondary injury, including both focal and diffuse brain injuries. Secondary injuries are multiple, parallel, interacting and interdependent cascades of biological reactions arising from discrete subcellular processes (e.g., toxicity due to reactive oxygen species, overstimulation of glutamate receptors, excessive influx of calcium and inflammatory upregulation) which are caused or exacerbated by the inflammatory response and progress after the initial (primary) injury. Abnormal calcium homeostasis is believed to be a critical component of the progression of secondary injury in both grey and white matter. For a review of TBI, see Park et al., CMAJ (2008) 178(9):1163-1170, the contents of which are incorporated herein in their entirety. Studies have shown that the cAMP-PICA signaling cascade is downregulated after TBI and treatment of PDE IV inhibitors such as rolipram to raise or restore cAMP level improves histopathological outcome and decreases inflammation after TBI. As Compounds of the present invention is a PDE1 inhibitor, it is believed that these compounds are also useful for the treatment of TBI, e.g., by restoring cAMP level and/or calcium homeostasis after traumatic brain injury.

The present invention also provides

-   -   (i) a Compound of the Invention, e.g., Formula I or any of         1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of         2.1-2.13, in free or pharmaceutically acceptable salt form, for         use as a pharmaceutical, for example for use in any method or in         the treatment of any disease or condition as hereinbefore set         forth,     -   (ii) the use of a Compound of the Invention, e.g., Formula I or         any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g.,         any of 2.1-2.13, in free or pharmaceutically acceptable salt         form, in the manufacture of a medicament for treating any         disease or condition as hereinbefore set forth,     -   (iii) a pharmaceutical composition comprising a Compound of the         Invention, e.g., Formula I or any of 1.1-1.84, or a Compound of         Formula II-A or II-B, e.g., any of 2.1-2.13, in free or         pharmaceutically acceptable salt form, in combination or         association with a pharmaceutically acceptable diluent or         carrier, and     -   (iv) a pharmaceutical composition comprising a Compound of the         Invention, e.g., Formula I or any of 1.1-1.84, or a Compound of         Formula II-A or II-B, e.g., any of 2.1Error! Reference source         not found.-2.13, in free or pharmaceutically acceptable salt         form, in combination or association with a pharmaceutically         acceptable diluent or carrier for use in the treatment of any         disease or condition as hereinbefore set forth.

Therefore, the invention provides use of a Compound of the Invention, e.g., Formula I or any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt or prodrug form, or a Compound of the Invention in a pharmaceutical composition form, for the manufacture of a medicament for the treatment or prophylactic treatment of the following diseases: Parkinson's disease, restless leg, tremors, dyskinesias, Huntington's disease, Alzheimer's disease, and drug-induced movement disorders; depression, attention deficit disorder, attention deficit hyperactivity disorder, bipolar illness, anxiety, sleep disorder, narcolepsy, cognitive impairment, dementia, Tourette's syndrome, autism, fragile X syndrome, psychostimulant withdrawal, and/or drug addiction; cerebrovascular disease, stroke, congestive heart disease, hypertension, pulmonary hypertension, and/or sexual dysfunction; asthma, chronic obstructive pulmonary disease, and/or allergic rhinitis, as well as autoimmune and inflammatory diseases; and/or female sexual dysfunction, exercise amenorrhoea, anovulation, menopause, menopausal symptoms, hypothyroidism, pre-menstrual syndrome, premature labor, infertility, irregular menstrual cycles, abnormal uterine bleeding, osteoporosis, multiple sclerosis, prostate enlargement, prostate cancer, hypothyroidism, estrogen-induced endometrial hyperplasia or carcinoma; and/or any disease or condition characterized by low levels of cAMP and/or cGMP (or inhibition of cAMP and/or cGMP signaling pathways) in cells expressing PDE1, and/or by reduced dopamine D1 receptor signaling activity; and/or any disease or condition that may be ameliorated by the enhancement of progesterone signaling;

The invention also provides use of a Compound of the Invention, e.g., a Compound of Formula I-A or I-B, e.g., any of 1.1-1.84, or a Compound of Formula II-A or II-B, e.g., any of 2.1-2.13, in free or pharmaceutically acceptable salt form, for the manufacture of a medicament for the treatment or prophylactic treatment of:

-   -   a) glaucoma or elevated intraocular pressure,     -   b) psychosis, for example, any conditions characterized by         psychotic symptoms such as hallucinations, paranoid or bizarre         delusions, or disorganized speech and thinking, e.g.,         schizophrenia, schizoaffective disorder, schizophreniform         disorder, psychotic disorder, delusional disorder, and mania,         such as in acute manic episodes and bipolar disorder,     -   c) traumatic brain injury.

The words “treatment” and “treating” are to be understood accordingly as embracing prophylaxis and treatment or amelioration of symptoms of disease as well as treatment of the cause of the disease.

For methods of treatment, the word “effective amount” is intended to encompass a therapeutically effective amount to treat a specific disease or disorder.

The term “pulmonary hypertension” is intended to encompass pulmonary arterial hypertension.

The term “patient” include human or non-human (i.e., animal) patient. In particular embodiment, the invention encompasses both human and nonhuman. In another embodiment, the invention encompasses nonhuman. In other embodiment, the term encompasses human.

The term “comprising” as used in this disclosure is intended to be open-ended and does not exclude additional, unrecited elements or method steps.

Compounds of the Invention are in particular useful for the treatment of Parkinson's disease, narcolepsy and female sexual dysfunction.

Compounds of the Invention, e.g., Formula I-A or I-B or any of 1.1-1.84, or II-A or II-B, any of 2.1-2.13, in free or pharmaceutically acceptable salt form may be used as a sole therapeutic agent, but may also be used in combination or for co-administration with other active agents. For example, as Compounds of the Invention potentiate the activity of D1 agonists, such as dopamine, they may be simultaneously, sequentially, or contemporaneously administered with conventional dopaminergic medications, such as levodopa and levodopa adjuncts (carbidopa, COMT inhibitors, MAO-B inhibitors), dopamine agonists, and anticholinergics, e.g., in the treatment of a patient having Parkinson's disease. In addition, the novel PDE 1 inhibitors, e.g., as described herein, may also be administered in combination with estrogen/estradiol/estriol and/or progesterone/progestins to enhance the effectiveness of hormone replacement therapy or treatment of estrogen-induced endometrial hyperplasia or carcinoma.

Dosages employed in practicing the present invention will of course vary depending, e.g. on the particular disease or condition to be treated, the particular Compound of the Invention used, the mode of administration, and the therapy desired. Compounds of the Invention may be administered by any suitable route, including orally, parenterally, transdermally, or by inhalation, but are preferably administered orally. In general, satisfactory results, e.g. for the treatment of diseases as hereinbefore set forth are indicated to be obtained on oral administration at dosages of the order from about 0.01 to 2.0 mg/kg. In larger mammals, for example humans, an indicated daily dosage for oral administration will accordingly be in the range of from about 0.75 to 150 mg, conveniently administered once, or in divided doses 2 to 4 times, daily or in sustained release form. Unit dosage forms for oral administration thus for example may comprise from about 0.2 to 75 or 150 mg, e.g. from about 0.2 or 2.0 to 50, 75 or 100 mg of a Compound of the Invention, together with a pharmaceutically acceptable diluent or carrier therefor.

Pharmaceutical compositions comprising Compounds of the Invention may be prepared using conventional diluents or excipients and techniques known in the galenic art. Thus oral dosage forms may include tablets, capsules, solutions, suspensions and the like.

EXAMPLES

The synthetic methods for various Compounds of the Present Invention are illustrated below. Other compounds of the Invention and their salts may be made using the methods as similarly described below and/or by methods similar to those generally described in the detailed description and by methods known in the chemical art.

Example 1 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

1) 2-((1R,2R)-2-hydroxycyclopentylamino)-3-methylpyrimidin-4(3H)-one

3-Methyluracil (12.6 mg, 0.1 mmol) was dissolved in 0.5 mL of DMF, and then BOP (71 mg, 0.16 mmol) was added. The mixture was stirred at room temperature for two minutes, then (1R,2R)-(−)-2-hydroxycyclopentylamine hydrochloride salt (22 mg, 0.16 mmol) was added, followed by DBU (51 uL, 3.4 mmol). The mixture was stirred at room temperature overnight. The reaction mixture was purified by a semi-preparative HPLC to give pure product (16 mg, yield 76%). MS (ESI) m/z 210.1 [M+H]⁺.

2) (3aS,8aR)-7-Methyl-1,2,3,3a,7,8a-hexahydro-3b,7,8-triaza-cyclopenta[a]inden-6-one

To a solution of 2-((1R,2R)-2-hydroxycyclopentylamino)-3-methylpyrimidin-4(3H)-one (130 mg, 0.62 mmol) in anhydrous THF (2 mL) is added triphenylphosphine (163 mg, 0.62 mmol). Five minutes later, diethyl azodicarboxylate (DEAD, 0.45 mL, 0.93 mmol) in toluene is added dropwise. The mixture is stirred at room temperature for 2 hours. Solvent is removed under vacuum, the residue is treated with 0.02 N HCl (40 mL). The precipitate is filtered off, and the filtrate is washed with CH₂Cl₂. The aqueous phase is evaporated to dryness under high vacuum to give product as solids (108 mg, yield 92%), which is used for the next reaction without further purification. MS (ESI) m/z 192.1 [M+H]⁺.

3) (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

Sodium hydride (95%, 112 mg, 4.44 mmol) is suspended in 3 mL of anhydrous THF, and then a mixture of (3aS,8aR)-7-Methyl-1,2,3,3a,7,8a-hexahydro-3b,7,8-triaza-cyclopenta[a]inden-6-one (283 mg, 1.48 mmol) and p-toluenesulfonylmethyl isocyanide (97%, 347 mg, 1.77 mmol) in 5 mL of anhydrous THF is added dropwise. The mixture is stirred at room temperature for an hour, and then quenched with water. The mixture is extracted with CH₂Cl₂ (5×10 mL). The combined organic phase is washed with brine, and then dried with anhydrous Na₂SO₄. After filtration, the filtrate is evaporated to dryness under reduced pressure to give crude product (320 mg, yield 94%) as brown solids, which is used for the next reaction without further purification. MS (ESI) m/z 231.1 [M+H]⁺.

4) (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

A suspension of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (140 mg, 0.61 mmol), 2-(4-(chloromethyl)phenyl)pyridine (0.12 g, 0.61 mmol) and cesium carbonate (400 mg, 1.22 mmol) in anhydrous DMF is stirred at room temperature overnight. The mixture is filtered through a 0.2 μL microfilter. The filtrate is purified by a semi-preparative HPLC to give 41 mg of pure product as off white solids. MS (ESI) m/z 398.2 [M+H]⁺.

Example 2 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2-(4-(6-fluoropyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 1 wherein 2-(4-(chloromethyl)phenyl)-6-fluoropyridine is used in step 4 instead of 2-(4-(chloromethyl)phenyl)pyridine. MS (ESI) m/z 416.2 [M+H]⁺.

Example 3 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-1,5-dimethyl-2-(4-(6-fluoropyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 1 wherein 1-(1-isocyanoethylsulfonyl)-4-methylbenzene is used in step 3 instead of p-toluenesulfonylmethyl isocyanide, and 2-(4-(chloromethyl)phenyl)-6-fluoropyridine is used in step 4 instead of 2-(4-(chloromethyl)phenyl)pyridine. MS (ESI) m/z 416.2 [M+H]⁺.

Example 4 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-1-chloro-5-methyl-2-(4-(6-fluoropyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

(6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2-(4-(6-fluoropyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (38 mg, 0.082 mmol) is dissolved in a mixture of CCl₄ and DMF (8/1, v/v). The solution is cooled to 0° C., and then a solution of N-chlorosuccinimide (10.9 mg, 0.082 mmol) in CCl₄ and DMF (8/1, v/v) is added dropwise. The reaction mixture is stirred at room temperature for half an hour. Solvents are removed under vacuum, and the residue is purified by a semi-preparative HPLC to give pure product as off white solids (16.5 mg, yield 45%). MS (ESI) m/z 450.1 [M+H]⁺.

Example 5 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-1-(phenylamino)-2-(4-(6-fluoropyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

Crude (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-1-chloro-5-methyl-2-(4-(6-fluoropyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (approx. 0.03 mmol) is dissolved in anhydrous CH₂Cl₂, and then trichloroacetic acid (5.2 mg, 0.03 mmol) is added, followed by aniline (5.8 uL, 0.06 mmol). The reaction mixture is heated in a Biotage microwave instrument at 100° C. for 2 hours. The mixture is purified by a semi-preparative HPLC to give 2.2 mg of product as solids. MS (ESI) m/z 507.2 [M+H]⁺.

Example 6 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2-(4-methoxy-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 1 wherein 1-(chloromethyl)-4-methoxybenzene is used in step 4 instead of 2-(4-(chloromethyl)phenyl)pyridine. MS (ESI) m/z 351.2 [M+H]⁺.

Example 7 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-(4-methoxy-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

1.0M LiHMDS in THF (4.2 mL, 4.2 mmol) is added dropwise to a solution of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2-(4-methoxy-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (500 mg, 1.4 mmol) and hexachloroethane (1.69 g, 7.13 mmol) at room temperature under argon. After 30 min, the mixture is quenched with saturated ammonium chloride aqueous solution at 0° C., and then basified with saturated sodium bicarbonate aqueous solution, followed by extractions with methylene chloride. The collected organic phase is washed with brine, dried over anhydrous sodium sulfate, and then evaporated to dryness under reduced pressure. The obtained crude product is purified by silica gel flash chromatography to give 165 mg of pure product as off white solid (yield: 30%). MS (ESI) m/z 385.2 [M+H]⁺.

Example 8 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

To a solution of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-(4-methoxy-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (95 mg, 0.25 mmol) in CH₂Cl₂ is slowly added TFA and trifluoromethanesulfonic acid (TFMSA). The mixture is stirred at room temperature overnight. Solvents and TFA are removed under reduced pressure. The residue is neutralized and dissolved in DMF, and then purified by a semi-preparative HPLC to give 77 mg of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one. A suspension of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (79 mg, 0.3 mmol), 2-(4-(chloromethyl)phenyl)pyridine (61 mg, 0.3 mmol) and cesium carbonate (192 mg, 0.6 mmol) in anhydrous DMF is stirred at room temperature for 4 h. The mixture is filtered through a 0.2 μL microfilter. The filtrate is purified by a semi-preparative HPLC to give pure product. MS (ESI) m/z 432.2 [M+H]⁺.

Example 9 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylamino)-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

(6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (5.6 mg, 0.013 mmol) is placed in a Biotage microwave tube, and then aniline (0.2 mL) is added. The mixture is heated at 150° C. for an hour. The mixture is purified by a semi-preparative HPLC to give product. MS (ESI) m/z 489.3 [M+H]⁺.

Example 10 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylamino)-2-(4-methoxy-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 9 wherein (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-(4-methoxy-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one is used instead of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one. MS (ESI) m/z 442.2 [M+H]⁺.

Example 11 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylamino)-2-(4-(1H-1,2,4-triazol-1-yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 9 wherein (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-(4-(1H-1,2,4-triazol-1-yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one is used instead of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one. MS (ESI) m/z 479.3 [M+H]⁺.

Example 12 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-1-(phenylamino)-2-(4-(pyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 9 wherein (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-1-chloro-2-(4-(pyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one is used instead of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one. MS (ESI) m/z 489.2 [M+H]⁺.

Example 13 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylamino)-2-(4-(pyridin-4-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 9 wherein (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-chloro-2-(4-(pyridin-4-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one is used instead of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one. MS (ESI) m/z 489.3 [M+H]⁺.

Example 14 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylamino)-2-(4-(1H-imidazol-1-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

The synthetic procedure of this compound is analogous to EXAMPLE 9 wherein (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-chloro-2-(4-(1H-imidazol-1-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one is used instead of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-3-chloro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one. MS (ESI) m/z 478.2 [M+H]⁺.

Example 15 (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylthio)-2-(4-(pyridin-2-yl)benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one

(6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2-((4-Pyridin-2yl)-benzyl)-cyclopent[4,5]imidazo[1,2-a]pyrrolo[4,3-e]pyrimidin-4(2H)-one (20 mg, 0.05 mmol) and phenyl disulfide (22 mg, 0.10 mmol) are dissolved in 400 μL of anhydrous THF, and then 1.0 M LiHMDS in THF (150 μL, 0.15 mmol) is added dropwise. The mixture is stirred at room temperature for 10 min, and then quenched with ammonium chloride aqueous solution. The mixture is diluted with DMF, and then purified by a semi-preparative HPLC to give pure product as pale yellow solid. MS (ESI) m/z 506.2 [M+H]⁺.

Example 16 Measurement of PDE1B Inhibition In Vitro Using IMAP Phosphodiesterase Assay Kit

Phosphodiesterase 1B (PDE1B) is a calcium/calmodulin dependent phosphodiesterase enzyme that converts cyclic guanosine monophosphate (cGMP) to 5′-guanosine monophosphate (5′-GMP). PDE1B can also convert a modified cGMP substrate, such as the fluorescent molecule cGMP-fluorescein, to the corresponding GMP-fluorescein. The generation of GMP-fluorescein from cGMP-fluorescein can be quantitated, using, for example, the IMAP (Molecular Devices, Sunnyvale, Calif.) immobilized-metal affinity particle reagent.

Briefly, the IMAP reagent binds with high affinity to the free 5′-phosphate that is found in GMP-fluorescein and not in cGMP-fluorescein. The resulting GMP-fluorescein—IMAP complex is large relative to cGMP-fluorescein. Small fluorophores that are bound up in a large, slowly tumbling, complex can be distinguished from unbound fluorophores, because the photons emitted as they fluoresce retain the same polarity as the photons used to excite the fluorescence.

In the phosphodiesterase assay, cGMP-fluorescein, which cannot be bound to IMAP, and therefore retains little fluorescence polarization, is converted to GMP-fluorescein, which, when bound to IMAP, yields a large increase in fluorescence polarization (Δmp). Inhibition of phosphodiesterase, therefore, is detected as a decrease in Δmp.

Enzyme Assay

-   Materials: All chemicals are available from Sigma-Aldrich (St.     Louis, Mo.) except for IMAP reagents (reaction buffer, binding     buffer, FL-GMP and IMAP beads), which are available from Molecular     Devices (Sunnyvale, Calif.). -   Assay: 3′,5′-cyclic-nucleotide-specific bovine brain     phosphodiesterase (Sigma, St. Louis, Mo.) is reconstituted with 50%     glycerol to 2.5 U/ml. One unit of enzyme will hydrolyze 1.0 μmole of     3′,5′-cAMP to 5′-AMP per min at pH 7.5 at 30° C. One part enzyme is     added to 1999 parts reaction buffer (30 μM CaCl₂, 10 U/ml of     calmodulin (Sigma P2277), 10 mM Tris-HCl pH 7.2, 10 mM MgCl₂, 0.1%     BSA, 0.05% NaN₃) to yield a final concentration of 1.25 mU/ml. 99 μl     of diluted enzyme solution is added into each well in a flat bottom     96-well polystyrene plate to which 1 μl of test compound dissolved     in 100% DMSO is added. The compounds are mixed and pre-incubated     with the enzyme for 10 min at room temperature.

The FL-GMP conversion reaction is initiated by combining 4 parts enzyme and inhibitor mix with 1 part substrate solution (0.225 μM) in a 384-well microtiter plate. The reaction is incubated in dark at room temperature for 15 min. The reaction is halted by addition of 60 μl of binding reagent (1:400 dilution of IMAP beads in binding buffer supplemented with 1:1800 dilution of antifoam) to each well of the 384-well plate. The plate is incubated at room temperature for 1 hour to allow IMAP binding to proceed to completion, and then placed in an Envision multimode microplate reader (PerkinElmer, Shelton, Conn.) to measure the fluorescence polarization (Δmp).

A decrease in GMP concentration, measured as decreased Δmp, is indicative of inhibition of PDE activity. IC₅₀ values are determined by measuring enzyme activity in the presence of 8 to 16 concentrations of compound ranging from 0.0037 nM to 80,000 nM and then plotting drug concentration versus ΔmP, which allows IC₅₀ values to be estimated using nonlinear regression software (XLFit; IDBS, Cambridge, Mass.).

The Compounds of the Invention may be tested in an assay as described or similarly described herein for PDE1 inhibitory activity. The exemplified compounds generally have IC₅₀ values of less than 100 μM, some less than 10 μM, some less than 500 nM, some less than 10 nM, some against PDE1A. the Compounds of Examples 1, 3 and 5 generally have IC₅₀ values of about or less than 10 μM, some less than 500 nM, some less than 10 nM, particularly against PDE1A.

Example 17 PDE1 Inhibitor Effect on Sexual Response in Female Rats

The effect of PDE1 inhibitors on Lordosis Response in female rats may be measured as described in Mani, et al., Science (2000) 287: 1053. Ovariectomized and cannulated wild-type rats are primed with 2 μg estrogen followed 24 hours later by intracerebroventricular (icy) injection of progesterone (2 μg), PDE1 inhibitors of the present invention (0.1 mg, 1.0 mg or 2.5 mg) or sesame oil vehicle (control). The rats are tested for lordosis response in the presence of male rats. Lordosis response is quantified by the lordosis quotient (LQ=number of lordosis/10 mounts×100). 

What is claimed is:
 1. An optionally substituted 4,5,7,8-tetrahydro-(optionally 4-oxo, 4-thioxo or 4-imino)-2H-imidazo[1,2-a]pyrrolo[3,4-e]pyrimidine or 4,5,7,8,9-pentahydro-(optionally 4-oxo, 4-thioxo or 4-imino)-2H-pyrimido[1,2-a]pyrrolo[3,4-e]pyrimidine, in free or salt form.
 2. The compound according to claim 1, wherein said compound is a Compound of Formula II-A or II-B

wherein (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂; (ii) L is a single bond, —N(H)—, —CH₂—, —S—, —S(O)— or —S(O₂)—; (iii) R₁ is H or C₁₋₄ alkyl; (iv) R₄ is H or C₁₋₆ alkyl and R₂ and R₃ are, independently, H C₁₋₆alkyl optionally substituted with halo or hydroxy, aryl, heteroaryl, (optionally hetero)arylalkoxy, (optionally hetero)arylC₁₋₆alkyl, R₂ and R₃ together form a 3- to 6-membered ring; or R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is a) -D-E-F, wherein: D is C₁₋₄alkylene; E is a single bond, C₂₋₄alkynylene, arylene or heteroarylene; F is H, aryl, heteroaryl, halo, haloC₁₋₄alkyl, —N(R₁₆)(R₁₇), or C₃₋₇cycloalkyl optionally containing at least one atom selected from a group consisting of N or O; wherein D, E and F are independently and optionally substituted with one or more halo, C₁₋₄alkyl, haloC₁₋₄alkyl, C₁₋₄alkoxy, hydroxy, carboxy, or an additional aryl or heteroaryl; or b) a haloC₁₋₄alkyl substituted heteroarylalkyl; or c) attached to the nitrogen on the pyrrolo portion of Formula II-A or II-B and is a moiety of Formula A

wherein X, Y and Z are, independently, N or C, and R₈, R₉, R₁₁ and R₁₂ are independently H or halogen, and R₁₀ is halogen, C₁₋₄alkyl, haloC₁₋₄alkyl, C₁₋₄alkoxy, C₃₋₇cycloalkyl, heteroC₃₋₇cycloalkyl, C₁₋₄haloalkyl, aryl, heteroaryl, arylcarbonyl, alkylsulfonyl, heteroarylcarbonyl, or alkoxycarbonyl; wherein the aryl, heteroaryl, cycloalkyl or heterocycloalkyl is independently, optionally substituted with one or more C₁₋₄alkyl, halogen, haloC₁₋₄alkyl, hydroxy, carboxy, —SH or an additional aryl or heteroaryl, provided that when X, Y, or Z is nitrogen, R₈, R₉, or R₁₀, respectively, is not present; (vi) R₆ is H, C₁₋₄alkyl, C₃₋₇cycloalkyl, heteroC₃₋₇cycloalkyl, aryl, heteroaryl, arylC₁₋₄alkyl, when L is a single bond, —CH₂—, —N(H)—, —S—, —S(O)— or S(O₂)—, wherein the aryl and heteroaryl are optionally substituted with one or more C₁₋₄alkyl, halogen, haloC₁₋₄alkyl, hydroxy, carboxy, or an additional aryl, heteroaryl or C₃₋₈cycloalkyl; or R₆ is H, arylamino, heteroarylamino, N,N-diC₁₋₄alkylamino, N,N-diarylamino, N-aryl-N-(arylC₁₋₄alkyl)amino, or —N(R₁₈)(R₁₉), when L is a single bond or —CH₂—, (vii) wherein the aryl and heteroaryl are optionally substituted with one or more C₁₋₄alkyl, halogen, haloC₁₋₄alkyl, hydroxy, carboxy, or an additional aryl, heteroaryl or C₃₋₈cycloalkyl; (viii) R₇ is H, C₁₋₆alkyl, halogen, —N(R₁₈)(R₁₉), hydroxy or C₁₋₆alkoxy; (ix) n=0 or 1; (x) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R₁₄, are, independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally hetero)arylC₁₋₄alkoxy, (optionally hetero)arylC₁₋₄alkyl or R₁₃ or R₁₄ can form a bridge with R₂ or R₄; (xi) R₁₅ is C₁₋₄alkyl, haloC₁₋₄alkyl, —OH or —OC₁₋₄alkyl; (xii) R₁₆ and R₁₇ are independently H or C₁₋₄alkyl; (xiii) R₁₈ and R₁₉ are independently H, C₁₋₄alkyl, C₃₋₈cycloalkyl, heteroC₃₋₈cycloalky, aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substituted with one or more halo, hydroxy, C₁₋₄alkyl, haloC₁₋₄alkyl, carboxy, or an additional aryl, heteroaryl or C₃₋₈cycloalkyl, (xiv) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl; in free or salt form.
 3. A Compound of Formula I-A or I-B

wherein (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂; (ii) L is a single bond, —N(H)—, —CH₂—, —S—, —S(O)— or —S(O₂)—; (iii) R₁ is H or C₁₋₄ alkyl; (iv) R₄ is H or C₁₋₆ alkyl and R₂ and R₃ are, independently, H or C₁₋₆alkyl optionally substituted with halo or hydroxy, aryl, heteroaryl, (optionally hetero)arylalkoxy, or (optionally hetero)arylC₁₋₆alkyl; or R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is a) -D-E-F, wherein: D is C₁₋₄alkylene; E is a single bond, C₂₋₄alkynylene, arylene or heteroarylene; F is H, aryl, heteroaryl, halo, haloC₁₋₄alkyl, —C(O)—R₁₅, —N(R₁₆)(R₁₇), or C₃₋₇cycloalkyl optionally containing at least one atom selected from a group consisting of N or O; wherein D, E and F are independently and optionally substituted with one or more halo, C₁₋₄alkyl, haloC₁₋₄alkyl; or b) a haloalkyl substituted heteroarylalkyl; or c) attached to the nitrogen on the pyrrolo portion of Formula I-A or I-B and is a moiety of Formula A

wherein X, Y and Z are, independently, N or C, and R₈, R₉, R₁₁ and R₁₂ are independently H or halogen, and R₁₀ is halogen, C₁₋₄alkyl, C₃₋₇cycloalkyl, C₁₋₄haloalkyl, aryl, heteroaryl, arylcarbonyl, alkylsulfonyl, heteroarylcarbonyl, or alkoxycarbonyl; provided that when X, Y, or Z is nitrogen, R₈, R₉, or R₁₀, respectively, is not present; (vi) R₆ is H, C₁₋₄alkyl, C₃₋₇cycloalkyl, aryl, heteroaryl, arylC₁₋₄alkyl, arylamino, heteroarylamino, N,N-diC₁₋₄alkylamino, N,N-diarylamino, N-aryl-N-(arylC₁₋₄alkyl)amino, or —N(R₁₈)(R₁₉); wherein the aryl or heteroaryl is optionally substituted with one or more halo, hydroxy or C₁₋₆alkoxy; (vii) R₇ is H, C₁₋₆alkyl, halogen, or —N(R₁₈)(R₁₉); (viii) n=0 or 1; (ix) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R₁₄, are, independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally hetero)arylC₁₋₄alkoxy or (optionally hetero)arylC₁₋₄alkyl; (x) R₁₅ is C₁₋₄alkyl, haloC₁₋₄alkyl, —OH or —OC₁₋₄alkyl; (xi) R₁₆ and R₁₇ are independently H or C₁₋₄alkyl; (xii) R₁₈ and R₁₉ are independently H, C₁₋₄alky or aryl wherein said aryl is optionally substituted with one or more halo or hydroxy; (xiii) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl, in free or salt form.
 4. The compound according to claim 2, selected from any of the following:

in free or salt form.
 5. The compound according to claim 2, selected from any of the following:

in free or salt form.
 6. A pharmaceutical composition comprising a compound according to claim 2, in free or pharmaceutically acceptable salt form, in admixture with a pharmaceutically acceptable diluent or carrier.
 7. The pharmaceutical composition according to claim 6, wherein salt and the diluents or carrier are opthalmically acceptable.
 8. A method of treating any of the following conditions: Parkinson's disease, restless leg, tremors, dyskinesias, Huntington's disease, Alzheimer's disease, and drug-induced movement disorders; depression, attention deficit disorder, attention deficit hyperactivity disorder, bipolar illness, anxiety, sleep disorder, narcolepsy, cognitive impairment, dementia, Tourette's syndrome, autism, fragile X syndrome, psychostimulant withdrawal, and/or drug addiction; cerebrovascular disease, stroke, congestive heart disease, hypertension, pulmonary hypertension, and/or sexual dysfunction; asthma, chronic obstructive pulmonary disease, and/or allergic rhinitis, as well as autoimmune and inflammatory diseases; and/or female sexual dysfunction, exercise amenorrhoea, anovulation, menopause, menopausal symptoms, hypothyroidism, pre-menstrual syndrome, premature labor, infertility, irregular menstrual cycles, abnormal uterine bleeding, osteoporosis, multiple sclerosis, prostate enlargement, prostate cancer, hypothyroidism, estrogen-induced endometrial hyperplasia or carcinoma; and/or any disease or condition characterized by low levels of cAMP and/or cGMP (or inhibition of cAMP and/or cGMP signaling pathways) in cells expressing PDE1, and/or by reduced dopamine D1 receptor signaling activity; and/or any disease or condition that may be ameliorated by the enhancement of progesterone signaling; comprising administering an effective amount of a compound according to claim 2, to a patient in need of such treatment.
 9. The method of claim 8, wherein the condition is Parkinson's disease.
 10. The method of claim 8, wherein the condition is cognitive impairment.
 11. The method of claim 8, wherein the condition is narcolepsy.
 12. The method of claim 11 further comprising administering a compound or compounds selected from central nervous system stimulants, modafinil, antidepressants, and gamma hydroxybutyrate, to a patient in need thereof.
 13. The method of claim 8, wherein said condition is female sexual dysfunction.
 14. The method of claim 13, further comprising administering a compound or compounds selected from a group consisting of estradiol, estriol, estradiol esters, progesterone and progestins to a patient in need thereof.
 15. A method for the treatment of glaucoma or elevated intraocular pressure comprising topical administration of a therapeutically effective amount of a compound according to claim 2, in free or pharmaceutically acceptable salt form, in an opthalmically compatible carrier to the eye of a patient in need thereof.
 16. A method for the treatment of psychosis, schizophrenia, schizoaffective disorder, schizophreniform disorder, psychotic disorder, delusional disorder, and mania, such as in acute manic episodes and bipolar disorder, comprising administering a therapeutically effective amount of a compound according to claim 2, in free or pharmaceutically acceptable salt form, to a patient in need thereof.
 17. A method for the treatment of traumatic brain injury comprising administering to a patient in need thereof, a compound according to claim 2, in free or pharmaceutically acceptable salt form.
 18. A method for lengthening or enhancing growth of the eyelashes by administering an effective amount of a prostaglandin analogue concomitantly, simultaneously or sequentially with an effective amount of a compound according to claim 2, in free or salt form.
 19. The compound according to claim 2, wherein (i) Q is C(═O), C(═S), C(═N(R₂₀)) or CH₂; (ii) L is —N(H)—, —S—, —S(O)— or —S(O₂)—; (iii) R₁ is H or C₁₋₄ alkyl; (iv) R₄ is H or C₁₋₆ alkyl and R₂ and R₃ are, independently, H C₁₋₆alkyl optionally substituted with halo or hydroxy, aryl, heteroaryl, (optionally hetero)arylalkoxy, (optionally hetero)arylC₁₋₆alkyl, or R₂ and R₃ together form a 3- to 6-membered ring; or R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is attached to the nitrogen on the pyrrolo portion of Formula II-A or II-B and is a moiety of Formula A

wherein X, Y and Z are, independently, N or C, and R₈, R₉, R₁₁ and R₁₂ are independently H or halogen, and R₁₀ is C₁₋₄alkoxy, C₃₋₇cycloalkyl, heteroC₃₋₇cycloalkyl, aryl, heteroaryl, wherein the aryl, heteroaryl, cycloalkyl or heterocycloalkyl is independently, optionally substituted with one or more C₁₋₄alkyl, halogen, haloC₁₋₄alkyl, hydroxy, carboxy, —SH or an additional aryl or heteroaryl, provided that when X, Y, or Z is nitrogen, R₉, R₉, or R₁₀, respectively, is not present; (vi) R₆ is H, C₁₋₄alkyl, C₃₋₇cycloalkyl, heteroC₃₋₇cycloalkyl, aryl, heteroaryl, wherein the aryl and heteroaryl are optionally substituted with one or more C₁₋₄alkyl, halogen, haloC₁₋₄alkyl, hydroxy, carboxy, or an additional aryl, heteroaryl or C₃₋₈cycloalkyl; (vii) R₇ is H, C₁₋₆alkyl, halogen, —N(R₁₈)(R₁₉), hydroxy or C₁₋₆alkoxy; (viii) n=0 or 1; (ix) when n=1, A is —C(R₁₃R₁₄)—, wherein R₁₃ and R₁₄, are, independently, H or C₁₋₄alkyl, aryl, heteroaryl, (optionally hetero)arylC₁₋₄alkoxy, (optionally hetero)arylC₁₋₄alkyl or R₁₃ or R₁₄ can form a bridge with R₂ or R₄; (x) R₁₈ and R₁₉ are independently H, C₁₋₄alky, C₃₋₈cycloalky, heteroC₃₋₈cycloalky, aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substituted with one or more halo, hydroxy, C₁₋₄alkyl, haloC₁₋₄alkyl, carboxy, or an additional aryl, heteroaryl or C₃₋₈cycloalkyl; (xi) R₂₀ is H, C₁₋₄alkyl or C₃₋₇cycloalkyl; in free or salt form.
 20. The compound according to claim 2, which is the compound of Formula I-A,

wherein (i) Q is C(═O); (ii) L is a —N(H), CH₂ or S; (iii) R₁ is H or C₁₋₄ alkyl; (iv) R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is attached to the nitrogen on the pyrrolo portion of Formula I-A and is a moiety of Formula A

wherein X, Y and Z are, independently, N or C, and R₈, R₉, R₁₁ and R₁₂ are independently H or halogen, and R₁₀ is C₃₋₇cycloalkyl, heteroC₃₋₇cycloalkyl, aryl, or heteroaryl, wherein the aryl, heteroaryl, cycloalkyl or heterocycloalkyl is independently, optionally substituted with one or more C₁₋₄alkyl, halogen, haloC₁₋₄alkyl, hydroxy, carboxy, —SH or an additional aryl or heteroaryl, provided that when X, Y, or Z is nitrogen, R₈, R₉, or R₁₀, respectively, is not present; (vi) R₆ is aryl optionally substituted with one or more halo, hydroxy; (vii) R₇ is H; and (viii) n=0, in free or salt form.
 21. The compound according to claim 3, wherein: (i) Q is C(═O); (ii) L is a —N(H)—; (iii) R₁ is H or C₁₋₄ alkyl; (iv) R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is -D-E-F, wherein: D is methylene; E is phenylene; F is heteroaryl, (vi) R₆ is H or aryl optionally substituted with one or more halo, hydroxy or C₁₋₆alkoxy; (vii) R₇ is H; and (viii) n=0, in free or salt form.
 22. The compound according to claim 3, wherein: (i) Q is C(═O); (ii) L is a —N(H)—; (iii) R₁ is H or C₁₋₄ alkyl; (iv) R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is -D-E-F, wherein: D is methylene; E is phenylene; F is heteroaryl substituted with one or more halo, haloC₁₋₄alkyl or C₁₋₄alkyl, (vi) R₆ is H or aryl optionally substituted with one or more halo, hydroxy or C₁₋₆alkoxy; (vii) R₇ is H; and (viii) n=0, in free or salt form.
 23. The compound according to claim 3, wherein (i) Q is C(═O); (ii) L is a —N(H)—; (iii) R₁ is H or C₁₋₄ alkyl; (iv) R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is -D-E-F, wherein: D is methylene; E is phenylene; F is pyridyl; (vi) R₆ is H or aryl optionally substituted with one or more halo, hydroxy or C₁₋₆alkoxy; (vii) R₇ is H; and (viii) n=0, in free or salt form.
 24. The compound according to claim 2 selected from any of the following:

in free or salt form.
 25. The compound according to claim 2 selected from any of the following:

in free or salt form.
 26. The compound according to claim 2 wherein the compound is:

in free or salt form.
 27. The compound according to claim 2 wherein the compound is:

in free or salt form.
 28. The compound according to claim 2, which is formula I-A:

wherein Q is C(═O) and L is —N(H), CH₂ or S, R₁-R₇ are as defined in claim 2, in free or salt form.
 29. The compound according to claim 2, which is the compound:

wherein: (i) Q is C(═O); (ii) L is —N(H)—, —CH₂— or —S—; (iii) R1 is H or C₁₋₄alkyl; (iv) R₂ is H and R₃ and R₄ together form a di-, tri- or tetramethylene bridge; (v) R₅ is -D-E-F and D is methylene; E is phenylene; F is heteroaryl; (vi) R₆ is aryl optionally substituted with one or more halo, hydroxy or C₁₋₆alkoxy; (vii) R₇ is H; and (viii) n=0, in free or salt form.
 30. The compound according to claim 2, wherein F is pyridyl, in free or salt form.
 31. The compound according to claim 2 selected from:

in free or salt form. 